Glutamate currents in mammalian spinal neurons: resolution of a paradox

Gary L. Westbrook; Mark L. Mayer

doi:10.1016/0006-8993(84)91107-7

Glutamate currents in mammalian spinal neurons: resolution of a paradox

Gary L. Westbrook, Mark L. Mayer

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Mouse spinal cord neurons grown in tissue culture were impaled with a pair of microelectrodes containing 1 M CsCl and voltage-clamped. Membrane currents evoked by excitatory amino acids were studied over the potential range -70 to +20 mV. Glutamate currents behaved as though generated by simultaneous activation of two conductance mechanisms, one voltage-sensitive, the other conventional. Block of NMDA receptors with the competitive antagonist 2-APV removed the voltage-sensitive component of the glutamate response. These results help to explain the paradoxical lack of conductance-change previously reported for glutamate responses recorded in the mammalian CNS.

Original language	English (US)
Pages (from-to)	375-379
Number of pages	5
Journal	Brain research
Volume	301
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(84)91107-7
State	Published - Jun 3 1984
Externally published	Yes

Keywords

excitatory amino acids
glutamate
tissue culture
voltage-clamp

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(84)91107-7

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title = "Glutamate currents in mammalian spinal neurons: resolution of a paradox",

abstract = "Mouse spinal cord neurons grown in tissue culture were impaled with a pair of microelectrodes containing 1 M CsCl and voltage-clamped. Membrane currents evoked by excitatory amino acids were studied over the potential range -70 to +20 mV. Glutamate currents behaved as though generated by simultaneous activation of two conductance mechanisms, one voltage-sensitive, the other conventional. Block of NMDA receptors with the competitive antagonist 2-APV removed the voltage-sensitive component of the glutamate response. These results help to explain the paradoxical lack of conductance-change previously reported for glutamate responses recorded in the mammalian CNS.",

keywords = "excitatory amino acids, glutamate, tissue culture, voltage-clamp",

author = "Westbrook, {Gary L.} and Mayer, {Mark L.}",

note = "Funding Information: We thank Dr. P. G. Nelson for the privilege of working in his laboratory, Sandy Fitzgerald and Dr. P. B. Guthrie for their skills in preparing the cultures, and Professor J. S. Kelly for help with revision of the manuscript. M.L.M. is a Beit Memorial Research Fellow and thanks the Royal Society for a study visit award. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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doi = "10.1016/0006-8993(84)91107-7",

language = "English (US)",

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AU - Westbrook, Gary L.

AU - Mayer, Mark L.

N1 - Funding Information: We thank Dr. P. G. Nelson for the privilege of working in his laboratory, Sandy Fitzgerald and Dr. P. B. Guthrie for their skills in preparing the cultures, and Professor J. S. Kelly for help with revision of the manuscript. M.L.M. is a Beit Memorial Research Fellow and thanks the Royal Society for a study visit award. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1984/6/3

Y1 - 1984/6/3

N2 - Mouse spinal cord neurons grown in tissue culture were impaled with a pair of microelectrodes containing 1 M CsCl and voltage-clamped. Membrane currents evoked by excitatory amino acids were studied over the potential range -70 to +20 mV. Glutamate currents behaved as though generated by simultaneous activation of two conductance mechanisms, one voltage-sensitive, the other conventional. Block of NMDA receptors with the competitive antagonist 2-APV removed the voltage-sensitive component of the glutamate response. These results help to explain the paradoxical lack of conductance-change previously reported for glutamate responses recorded in the mammalian CNS.

AB - Mouse spinal cord neurons grown in tissue culture were impaled with a pair of microelectrodes containing 1 M CsCl and voltage-clamped. Membrane currents evoked by excitatory amino acids were studied over the potential range -70 to +20 mV. Glutamate currents behaved as though generated by simultaneous activation of two conductance mechanisms, one voltage-sensitive, the other conventional. Block of NMDA receptors with the competitive antagonist 2-APV removed the voltage-sensitive component of the glutamate response. These results help to explain the paradoxical lack of conductance-change previously reported for glutamate responses recorded in the mammalian CNS.

KW - excitatory amino acids

KW - glutamate

KW - tissue culture

KW - voltage-clamp

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Glutamate currents in mammalian spinal neurons: resolution of a paradox

Abstract

Keywords

ASJC Scopus subject areas

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