Glutamate currents in mammalian spinal neurons: resolution of a paradox

Gary Westbrook, Mark L. Mayer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Mouse spinal cord neurons grown in tissue culture were impaled with a pair of microelectrodes containing 1 M CsCl and voltage-clamped. Membrane currents evoked by excitatory amino acids were studied over the potential range -70 to +20 mV. Glutamate currents behaved as though generated by simultaneous activation of two conductance mechanisms, one voltage-sensitive, the other conventional. Block of NMDA receptors with the competitive antagonist 2-APV removed the voltage-sensitive component of the glutamate response. These results help to explain the paradoxical lack of conductance-change previously reported for glutamate responses recorded in the mammalian CNS.

Original languageEnglish (US)
Pages (from-to)375-379
Number of pages5
JournalBrain Research
Volume301
Issue number2
DOIs
StatePublished - Jun 3 1984
Externally publishedYes

Fingerprint

Glutamic Acid
Neurons
2-Amino-5-phosphonovalerate
Excitatory Amino Acids
Microelectrodes
N-Methyl-D-Aspartate Receptors
Spinal Cord
Membranes
cesium chloride

Keywords

  • excitatory amino acids
  • glutamate
  • tissue culture
  • voltage-clamp

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Glutamate currents in mammalian spinal neurons : resolution of a paradox. / Westbrook, Gary; Mayer, Mark L.

In: Brain Research, Vol. 301, No. 2, 03.06.1984, p. 375-379.

Research output: Contribution to journalArticle

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