Glutamate currents in mammalian spinal neurons: resolution of a paradox

Gary L. Westbrook, Mark L. Mayer

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Mouse spinal cord neurons grown in tissue culture were impaled with a pair of microelectrodes containing 1 M CsCl and voltage-clamped. Membrane currents evoked by excitatory amino acids were studied over the potential range -70 to +20 mV. Glutamate currents behaved as though generated by simultaneous activation of two conductance mechanisms, one voltage-sensitive, the other conventional. Block of NMDA receptors with the competitive antagonist 2-APV removed the voltage-sensitive component of the glutamate response. These results help to explain the paradoxical lack of conductance-change previously reported for glutamate responses recorded in the mammalian CNS.

Original languageEnglish (US)
Pages (from-to)375-379
Number of pages5
JournalBrain research
Volume301
Issue number2
DOIs
StatePublished - Jun 3 1984

Keywords

  • excitatory amino acids
  • glutamate
  • tissue culture
  • voltage-clamp

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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