Glucose starvation induces mutation and lineage-dependent adaptive responses in a large collection of cancer cell lines

Ningning He, Nayoung Kim, Euna Jeong, Yiling Lu, Gordon Mills, Sukjoon Yoon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tolerance of glucose deprivation is an important factor for cancer proliferation, survival, migration and progression. To systematically understand adaptive responses under glucose starvation in cancers, we analyzed reverse phase protein array (RPPA) data of 115 protein antibodies across a panel of approximately 170 heterogeneous cancer cell lines, cultured under normal and low glucose conditions. In general, glucose starvation broadly altered levels of many of the proteins and phosphoproteins assessed across the cell lines. Many mTOR pathway components were selectively sensitive to glucose stress, although the change in their levels still varied greatly across the cell line set. Furthermore, lineage-and genotype-based classification of cancer cell lines revealed mutation-specific variation of protein expression and phosphorylation in response to glucose starvation. Decreased AKT phosphorylation (S473) was significantly associated with PTEN mutation under glucose starvation conditions in lung cancer cell lines. The present study (see TCPAportal.org for data resource) provides insight into adaptive responses to glucose deprivation under diverse cellular contexts.

Original languageEnglish (US)
Pages (from-to)67-72
Number of pages6
JournalInternational Journal of Oncology
Volume48
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Starvation
Glucose
Cell Line
Mutation
Neoplasms
Phosphorylation
Protein Array Analysis
Proteins
Phosphoproteins
Lung Neoplasms
Genotype
Antibodies

Keywords

  • Cancer cell line panel
  • Glucose starvation
  • Mutation
  • Reverse phase protein array

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Glucose starvation induces mutation and lineage-dependent adaptive responses in a large collection of cancer cell lines. / He, Ningning; Kim, Nayoung; Jeong, Euna; Lu, Yiling; Mills, Gordon; Yoon, Sukjoon.

In: International Journal of Oncology, Vol. 48, No. 1, 01.01.2016, p. 67-72.

Research output: Contribution to journalArticle

He, Ningning ; Kim, Nayoung ; Jeong, Euna ; Lu, Yiling ; Mills, Gordon ; Yoon, Sukjoon. / Glucose starvation induces mutation and lineage-dependent adaptive responses in a large collection of cancer cell lines. In: International Journal of Oncology. 2016 ; Vol. 48, No. 1. pp. 67-72.
@article{b4d10cf7938249bfba0c595b5d6d5531,
title = "Glucose starvation induces mutation and lineage-dependent adaptive responses in a large collection of cancer cell lines",
abstract = "Tolerance of glucose deprivation is an important factor for cancer proliferation, survival, migration and progression. To systematically understand adaptive responses under glucose starvation in cancers, we analyzed reverse phase protein array (RPPA) data of 115 protein antibodies across a panel of approximately 170 heterogeneous cancer cell lines, cultured under normal and low glucose conditions. In general, glucose starvation broadly altered levels of many of the proteins and phosphoproteins assessed across the cell lines. Many mTOR pathway components were selectively sensitive to glucose stress, although the change in their levels still varied greatly across the cell line set. Furthermore, lineage-and genotype-based classification of cancer cell lines revealed mutation-specific variation of protein expression and phosphorylation in response to glucose starvation. Decreased AKT phosphorylation (S473) was significantly associated with PTEN mutation under glucose starvation conditions in lung cancer cell lines. The present study (see TCPAportal.org for data resource) provides insight into adaptive responses to glucose deprivation under diverse cellular contexts.",
keywords = "Cancer cell line panel, Glucose starvation, Mutation, Reverse phase protein array",
author = "Ningning He and Nayoung Kim and Euna Jeong and Yiling Lu and Gordon Mills and Sukjoon Yoon",
year = "2016",
month = "1",
day = "1",
doi = "10.3892/ijo.2015.3242",
language = "English (US)",
volume = "48",
pages = "67--72",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "1",

}

TY - JOUR

T1 - Glucose starvation induces mutation and lineage-dependent adaptive responses in a large collection of cancer cell lines

AU - He, Ningning

AU - Kim, Nayoung

AU - Jeong, Euna

AU - Lu, Yiling

AU - Mills, Gordon

AU - Yoon, Sukjoon

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Tolerance of glucose deprivation is an important factor for cancer proliferation, survival, migration and progression. To systematically understand adaptive responses under glucose starvation in cancers, we analyzed reverse phase protein array (RPPA) data of 115 protein antibodies across a panel of approximately 170 heterogeneous cancer cell lines, cultured under normal and low glucose conditions. In general, glucose starvation broadly altered levels of many of the proteins and phosphoproteins assessed across the cell lines. Many mTOR pathway components were selectively sensitive to glucose stress, although the change in their levels still varied greatly across the cell line set. Furthermore, lineage-and genotype-based classification of cancer cell lines revealed mutation-specific variation of protein expression and phosphorylation in response to glucose starvation. Decreased AKT phosphorylation (S473) was significantly associated with PTEN mutation under glucose starvation conditions in lung cancer cell lines. The present study (see TCPAportal.org for data resource) provides insight into adaptive responses to glucose deprivation under diverse cellular contexts.

AB - Tolerance of glucose deprivation is an important factor for cancer proliferation, survival, migration and progression. To systematically understand adaptive responses under glucose starvation in cancers, we analyzed reverse phase protein array (RPPA) data of 115 protein antibodies across a panel of approximately 170 heterogeneous cancer cell lines, cultured under normal and low glucose conditions. In general, glucose starvation broadly altered levels of many of the proteins and phosphoproteins assessed across the cell lines. Many mTOR pathway components were selectively sensitive to glucose stress, although the change in their levels still varied greatly across the cell line set. Furthermore, lineage-and genotype-based classification of cancer cell lines revealed mutation-specific variation of protein expression and phosphorylation in response to glucose starvation. Decreased AKT phosphorylation (S473) was significantly associated with PTEN mutation under glucose starvation conditions in lung cancer cell lines. The present study (see TCPAportal.org for data resource) provides insight into adaptive responses to glucose deprivation under diverse cellular contexts.

KW - Cancer cell line panel

KW - Glucose starvation

KW - Mutation

KW - Reverse phase protein array

UR - http://www.scopus.com/inward/record.url?scp=84953243014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84953243014&partnerID=8YFLogxK

U2 - 10.3892/ijo.2015.3242

DO - 10.3892/ijo.2015.3242

M3 - Article

C2 - 26573869

AN - SCOPUS:84953243014

VL - 48

SP - 67

EP - 72

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 1

ER -