Glucocorticoids Induce Cytosolic Phospholipase A2 and Prostaglandin H Synthase Type 2 but Not Microsomal Prostaglandin E Synthase (PGES) and Cytosolic PGES Expression in Cultured Primary Human Amnion Cells

Kang Sun, Runlin Ma, Xiaolan Cui, Begoña Campos, Rose Webster, Diane Brockman, Leslie Myatt

Research output: Contribution to journalArticle

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Abstract

This study examines the regulation of major enzymes in prostaglandin E 2 (PGE2) synthesis by glucocorticoids in separate cultures of human amnion epithelial and fibroblast cells at term. Cytosolic phospholipase A2 (cPLA2), cytosolic PGES (cPGES), and microsomal PGES (mPGES) mRNA were expressed at similar levels in both cell types, whereas a greater prostaglandin H synthase type 2 (PGHS-2) mRNA expression was observed in amnion fibroblasts than in epithelial cells. Amnion fibroblasts produced 50-fold more PGE2 per cell than epithelial cells. Dexamethasone (0.01-1 μM) increased PGE2 production in amnion fibroblasts in a concentration-dependent manner but did not affect PGE2 production in amnion epithelial cells. Both mRNA and protein expression of cPLA2 and PGHS-2 but not cPGES and mPGES were increased in a dose-dependent manner by dexamethasone (0.01-1 μM) in amnion fibroblasts. Induction of cPLA2 and PGHS-2 mRNA by dexamethasone was blocked by RU486. Dexamethasone did not affect PGHS-2, cPGES, and mPGES mRNA expression in amnion epithelial cells. In conclusion, amnion fibroblasts express a higher level of PGHS-2 mRNA and produced more PGE2 per cell than amnion epithelial cells at term of human pregnancy. Glucocorticoids increase PGE2 production only in the amnion fibroblasts mainly through induction of cPLA2 and PGHS-2 expression.

Original languageEnglish (US)
Pages (from-to)5564-5571
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume88
Issue number11
DOIs
StatePublished - Nov 2003
Externally publishedYes

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Cytosolic Phospholipases A2
Amnion
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Prostaglandins E
Fibroblasts
Glucocorticoids
Messenger RNA
Dexamethasone
Epithelial Cells
Prostaglandin-E Synthases
Cell culture
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Glucocorticoids Induce Cytosolic Phospholipase A2 and Prostaglandin H Synthase Type 2 but Not Microsomal Prostaglandin E Synthase (PGES) and Cytosolic PGES Expression in Cultured Primary Human Amnion Cells. / Sun, Kang; Ma, Runlin; Cui, Xiaolan; Campos, Begoña; Webster, Rose; Brockman, Diane; Myatt, Leslie.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 88, No. 11, 11.2003, p. 5564-5571.

Research output: Contribution to journalArticle

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abstract = "This study examines the regulation of major enzymes in prostaglandin E 2 (PGE2) synthesis by glucocorticoids in separate cultures of human amnion epithelial and fibroblast cells at term. Cytosolic phospholipase A2 (cPLA2), cytosolic PGES (cPGES), and microsomal PGES (mPGES) mRNA were expressed at similar levels in both cell types, whereas a greater prostaglandin H synthase type 2 (PGHS-2) mRNA expression was observed in amnion fibroblasts than in epithelial cells. Amnion fibroblasts produced 50-fold more PGE2 per cell than epithelial cells. Dexamethasone (0.01-1 μM) increased PGE2 production in amnion fibroblasts in a concentration-dependent manner but did not affect PGE2 production in amnion epithelial cells. Both mRNA and protein expression of cPLA2 and PGHS-2 but not cPGES and mPGES were increased in a dose-dependent manner by dexamethasone (0.01-1 μM) in amnion fibroblasts. Induction of cPLA2 and PGHS-2 mRNA by dexamethasone was blocked by RU486. Dexamethasone did not affect PGHS-2, cPGES, and mPGES mRNA expression in amnion epithelial cells. In conclusion, amnion fibroblasts express a higher level of PGHS-2 mRNA and produced more PGE2 per cell than amnion epithelial cells at term of human pregnancy. Glucocorticoids increase PGE2 production only in the amnion fibroblasts mainly through induction of cPLA2 and PGHS-2 expression.",
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