Glucocorticoid receptor in polymorphonuclear leukocytes: A simple method for leukocyte glucocorticoid receptor characterization

Tohru Murakami, David Brandon, David Rodbard, Donald (Lynn) Loriaux, Mortimer B. Lipsett

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Glucocorticoid receptors of polymorphonuclear leukocytes (PMN) have been characterized and compared with those of mononuclear leukocytes (MNL). Cytosol receptors from PMN and MNL have similar binding affinities for [3H]-dexamethasone (Dex). nearly superimposable sedimentation profiles on sucrose density gradients, and similar elution patterns from DEAE-Sephadex. Thus, the glucocorticoid receptors of MNL and PMN have similar biochemical properties. We have developed a simple method for preparing leukocytes from 20 ml of blood to characterize and quantify the glucocorticoid receptor. The Scatchard analysis of [3H]-Dex binding to intact leukocytes shows a single class of binding sites; the apparent Kd was dependent on the duration of incubation, but the binding capacity remained constant after 2 h incubation.

Original languageEnglish (US)
Pages (from-to)475-481
Number of pages7
JournalJournal of Steroid Biochemistry
Volume10
Issue number5
DOIs
StatePublished - 1979
Externally publishedYes

Fingerprint

Mononuclear Leukocytes
Glucocorticoid Receptors
Neutrophils
Leukocytes
Dexamethasone
DEAE-Dextran
Sedimentation
Cytosol
Sucrose
Blood
Binding Sites

Keywords

  • (Dex)
  • 1,3,5(10)-estratriene-3,17β-diol
  • 11β 17, 21-trihydroxy-4-pregnene-3,20-dione
  • 16α,17,21-tetrahydroxy-1,4-pregnadiene-3
  • 17β-hydroxy-4-androsten-3-one
  • 17β-hydroxy-5α-androstan-3-one
  • 20-dione
  • 20-dione-16,17-acetonide
  • 21-hydroxy-4-pregnene-3
  • 3α-hydroxy-5α-androstan-17-one
  • 3β-hydroxy-5-androsten-17-one
  • 4-pregnene-3,20-dione
  • 9α-fluoro-11β
  • 9α-fluoro-16α-methyl-11β,17,21-trihydroxy-1,4- pregnadiene-3, 20-dione
  • aldosterone, 11, 21-dihydroxy-4-pregnene-3, 20-dione-18-al
  • cortisol
  • dehydroepiandrosterone
  • deoxycorticosterone
  • dexamethasone
  • dihydrotestosterone
  • estradiol (17β-estradiol)
  • etiocholanolone
  • MNL
  • mononuclear leukocytes
  • PMN
  • polymorphonuclear leukocytes
  • progesterone
  • testosterone
  • triamcinolone acetonide

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Glucocorticoid receptor in polymorphonuclear leukocytes : A simple method for leukocyte glucocorticoid receptor characterization. / Murakami, Tohru; Brandon, David; Rodbard, David; Loriaux, Donald (Lynn); Lipsett, Mortimer B.

In: Journal of Steroid Biochemistry, Vol. 10, No. 5, 1979, p. 475-481.

Research output: Contribution to journalArticle

@article{62c030ebe7134dfaa6b60a2b1866f1f9,
title = "Glucocorticoid receptor in polymorphonuclear leukocytes: A simple method for leukocyte glucocorticoid receptor characterization",
abstract = "Glucocorticoid receptors of polymorphonuclear leukocytes (PMN) have been characterized and compared with those of mononuclear leukocytes (MNL). Cytosol receptors from PMN and MNL have similar binding affinities for [3H]-dexamethasone (Dex). nearly superimposable sedimentation profiles on sucrose density gradients, and similar elution patterns from DEAE-Sephadex. Thus, the glucocorticoid receptors of MNL and PMN have similar biochemical properties. We have developed a simple method for preparing leukocytes from 20 ml of blood to characterize and quantify the glucocorticoid receptor. The Scatchard analysis of [3H]-Dex binding to intact leukocytes shows a single class of binding sites; the apparent Kd was dependent on the duration of incubation, but the binding capacity remained constant after 2 h incubation.",
keywords = "(Dex), 1,3,5(10)-estratriene-3,17β-diol, 11β 17, 21-trihydroxy-4-pregnene-3,20-dione, 16α,17,21-tetrahydroxy-1,4-pregnadiene-3, 17β-hydroxy-4-androsten-3-one, 17β-hydroxy-5α-androstan-3-one, 20-dione, 20-dione-16,17-acetonide, 21-hydroxy-4-pregnene-3, 3α-hydroxy-5α-androstan-17-one, 3β-hydroxy-5-androsten-17-one, 4-pregnene-3,20-dione, 9α-fluoro-11β, 9α-fluoro-16α-methyl-11β,17,21-trihydroxy-1,4- pregnadiene-3, 20-dione, aldosterone, 11, 21-dihydroxy-4-pregnene-3, 20-dione-18-al, cortisol, dehydroepiandrosterone, deoxycorticosterone, dexamethasone, dihydrotestosterone, estradiol (17β-estradiol), etiocholanolone, MNL, mononuclear leukocytes, PMN, polymorphonuclear leukocytes, progesterone, testosterone, triamcinolone acetonide",
author = "Tohru Murakami and David Brandon and David Rodbard and Loriaux, {Donald (Lynn)} and Lipsett, {Mortimer B.}",
year = "1979",
doi = "10.1016/0022-4731(79)90207-3",
language = "English (US)",
volume = "10",
pages = "475--481",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
issn = "0960-0760",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Glucocorticoid receptor in polymorphonuclear leukocytes

T2 - A simple method for leukocyte glucocorticoid receptor characterization

AU - Murakami, Tohru

AU - Brandon, David

AU - Rodbard, David

AU - Loriaux, Donald (Lynn)

AU - Lipsett, Mortimer B.

PY - 1979

Y1 - 1979

N2 - Glucocorticoid receptors of polymorphonuclear leukocytes (PMN) have been characterized and compared with those of mononuclear leukocytes (MNL). Cytosol receptors from PMN and MNL have similar binding affinities for [3H]-dexamethasone (Dex). nearly superimposable sedimentation profiles on sucrose density gradients, and similar elution patterns from DEAE-Sephadex. Thus, the glucocorticoid receptors of MNL and PMN have similar biochemical properties. We have developed a simple method for preparing leukocytes from 20 ml of blood to characterize and quantify the glucocorticoid receptor. The Scatchard analysis of [3H]-Dex binding to intact leukocytes shows a single class of binding sites; the apparent Kd was dependent on the duration of incubation, but the binding capacity remained constant after 2 h incubation.

AB - Glucocorticoid receptors of polymorphonuclear leukocytes (PMN) have been characterized and compared with those of mononuclear leukocytes (MNL). Cytosol receptors from PMN and MNL have similar binding affinities for [3H]-dexamethasone (Dex). nearly superimposable sedimentation profiles on sucrose density gradients, and similar elution patterns from DEAE-Sephadex. Thus, the glucocorticoid receptors of MNL and PMN have similar biochemical properties. We have developed a simple method for preparing leukocytes from 20 ml of blood to characterize and quantify the glucocorticoid receptor. The Scatchard analysis of [3H]-Dex binding to intact leukocytes shows a single class of binding sites; the apparent Kd was dependent on the duration of incubation, but the binding capacity remained constant after 2 h incubation.

KW - (Dex)

KW - 1,3,5(10)-estratriene-3,17β-diol

KW - 11β 17, 21-trihydroxy-4-pregnene-3,20-dione

KW - 16α,17,21-tetrahydroxy-1,4-pregnadiene-3

KW - 17β-hydroxy-4-androsten-3-one

KW - 17β-hydroxy-5α-androstan-3-one

KW - 20-dione

KW - 20-dione-16,17-acetonide

KW - 21-hydroxy-4-pregnene-3

KW - 3α-hydroxy-5α-androstan-17-one

KW - 3β-hydroxy-5-androsten-17-one

KW - 4-pregnene-3,20-dione

KW - 9α-fluoro-11β

KW - 9α-fluoro-16α-methyl-11β,17,21-trihydroxy-1,4- pregnadiene-3, 20-dione

KW - aldosterone, 11, 21-dihydroxy-4-pregnene-3, 20-dione-18-al

KW - cortisol

KW - dehydroepiandrosterone

KW - deoxycorticosterone

KW - dexamethasone

KW - dihydrotestosterone

KW - estradiol (17β-estradiol)

KW - etiocholanolone

KW - MNL

KW - mononuclear leukocytes

KW - PMN

KW - polymorphonuclear leukocytes

KW - progesterone

KW - testosterone

KW - triamcinolone acetonide

UR - http://www.scopus.com/inward/record.url?scp=0018761204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018761204&partnerID=8YFLogxK

U2 - 10.1016/0022-4731(79)90207-3

DO - 10.1016/0022-4731(79)90207-3

M3 - Article

C2 - 459498

AN - SCOPUS:0018761204

VL - 10

SP - 475

EP - 481

JO - Journal of Steroid Biochemistry and Molecular Biology

JF - Journal of Steroid Biochemistry and Molecular Biology

SN - 0960-0760

IS - 5

ER -