Glucocorticoid receptor in polymorphonuclear leukocytes: A simple method for leukocyte glucocorticoid receptor characterization

Tohru Murakami, David Brandon, David Rodbard, D. Lynn Loriaux, Mortimer B. Lipsett

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Glucocorticoid receptors of polymorphonuclear leukocytes (PMN) have been characterized and compared with those of mononuclear leukocytes (MNL). Cytosol receptors from PMN and MNL have similar binding affinities for [3H]-dexamethasone (Dex). nearly superimposable sedimentation profiles on sucrose density gradients, and similar elution patterns from DEAE-Sephadex. Thus, the glucocorticoid receptors of MNL and PMN have similar biochemical properties. We have developed a simple method for preparing leukocytes from 20 ml of blood to characterize and quantify the glucocorticoid receptor. The Scatchard analysis of [3H]-Dex binding to intact leukocytes shows a single class of binding sites; the apparent Kd was dependent on the duration of incubation, but the binding capacity remained constant after 2 h incubation.

Original languageEnglish (US)
Pages (from-to)475-481
Number of pages7
JournalJournal of Steroid Biochemistry
Volume10
Issue number5
DOIs
StatePublished - May 1979

Keywords

  • (Dex)
  • 1,3,5(10)-estratriene-3,17β-diol
  • 11β 17, 21-trihydroxy-4-pregnene-3,20-dione
  • 16α,17,21-tetrahydroxy-1,4-pregnadiene-3
  • 17β-hydroxy-4-androsten-3-one
  • 17β-hydroxy-5α-androstan-3-one
  • 20-dione
  • 20-dione-16,17-acetonide
  • 21-hydroxy-4-pregnene-3
  • 3α-hydroxy-5α-androstan-17-one
  • 3β-hydroxy-5-androsten-17-one
  • 4-pregnene-3,20-dione
  • 9α-fluoro-11β
  • 9α-fluoro-16α-methyl-11β,17,21-trihydroxy-1,4- pregnadiene-3, 20-dione
  • MNL
  • PMN
  • aldosterone, 11, 21-dihydroxy-4-pregnene-3, 20-dione-18-al
  • cortisol
  • dehydroepiandrosterone
  • deoxycorticosterone
  • dexamethasone
  • dihydrotestosterone
  • estradiol (17β-estradiol)
  • etiocholanolone
  • mononuclear leukocytes
  • polymorphonuclear leukocytes
  • progesterone
  • testosterone
  • triamcinolone acetonide

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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