Abstract
Glucocorticoid receptors of polymorphonuclear leukocytes (PMN) have been characterized and compared with those of mononuclear leukocytes (MNL). Cytosol receptors from PMN and MNL have similar binding affinities for [3H]-dexamethasone (Dex). nearly superimposable sedimentation profiles on sucrose density gradients, and similar elution patterns from DEAE-Sephadex. Thus, the glucocorticoid receptors of MNL and PMN have similar biochemical properties. We have developed a simple method for preparing leukocytes from 20 ml of blood to characterize and quantify the glucocorticoid receptor. The Scatchard analysis of [3H]-Dex binding to intact leukocytes shows a single class of binding sites; the apparent Kd was dependent on the duration of incubation, but the binding capacity remained constant after 2 h incubation.
Original language | English (US) |
---|---|
Pages (from-to) | 475-481 |
Number of pages | 7 |
Journal | Journal of Steroid Biochemistry |
Volume | 10 |
Issue number | 5 |
DOIs | |
State | Published - May 1979 |
Externally published | Yes |
Keywords
- (Dex)
- 1,3,5(10)-estratriene-3,17β-diol
- 11β 17, 21-trihydroxy-4-pregnene-3,20-dione
- 16α,17,21-tetrahydroxy-1,4-pregnadiene-3
- 17β-hydroxy-4-androsten-3-one
- 17β-hydroxy-5α-androstan-3-one
- 20-dione
- 20-dione-16,17-acetonide
- 21-hydroxy-4-pregnene-3
- 3α-hydroxy-5α-androstan-17-one
- 3β-hydroxy-5-androsten-17-one
- 4-pregnene-3,20-dione
- 9α-fluoro-11β
- 9α-fluoro-16α-methyl-11β,17,21-trihydroxy-1,4- pregnadiene-3, 20-dione
- MNL
- PMN
- aldosterone, 11, 21-dihydroxy-4-pregnene-3, 20-dione-18-al
- cortisol
- dehydroepiandrosterone
- deoxycorticosterone
- dexamethasone
- dihydrotestosterone
- estradiol (17β-estradiol)
- etiocholanolone
- mononuclear leukocytes
- polymorphonuclear leukocytes
- progesterone
- testosterone
- triamcinolone acetonide
ASJC Scopus subject areas
- Biochemistry
- Endocrinology