Glucocorticoid induction of β-adrenergic receptors in the DDT1 MF-2 smooth muscle cell line involves synthesis of new receptor

James S. Norris, Pamela Brown, Jeffrey Cohen, Lawrence E. Cornett, Peter O. Kohler, Stewart L. MacLeod, Keith Popovich, Robert B. Robey, Mark Sifford, Allen J. Syms, Roy G. Smith

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We have shown that glucocorticoids induce the appearance of β2-adrenergic receptors in membranes of the ductus deferens smooth muscle cell line (DDT1 MF-2). A concomitant increase in isoproterenol stimulated adenylate cyclase activity in the absence of exogenously applied GTP was observed as was a significantly increased (p < 0.05) sensitivity of the adenylate cyclase system to exogenously applied GTP. However, no significant difference in the maximal velocity of adenylate cyclase between control and steroid treatment was measurable in the presence of sodium fluoride. Induction of β2-adrenergic receptors in DDT1 MF-2 cells is correlated with the presence of steroid receptors (androgen and glucocorticoid) in the cells since estrogens and progesterones had no effect on receptor levels. Finally, utilizing dense amino acid labeling of cells to measure old versus newly synthesized receptor sites by a density shift method, we have documented that glucocorticoid induction of β2-adrenergic receptors involves synthesis of new receptor protein.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume74
Issue number1
DOIs
StatePublished - Mar 1 1987

Keywords

  • adenylate cyclase
  • glucocorticoid receptor
  • smooth muscle
  • β-adrenergic receptor regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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    Norris, J. S., Brown, P., Cohen, J., Cornett, L. E., Kohler, P. O., MacLeod, S. L., Popovich, K., Robey, R. B., Sifford, M., Syms, A. J., & Smith, R. G. (1987). Glucocorticoid induction of β-adrenergic receptors in the DDT1 MF-2 smooth muscle cell line involves synthesis of new receptor. Molecular and Cellular Biochemistry, 74(1), 21-27. https://doi.org/10.1007/BF00221909