Global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein B

Kenneth A. Simmen, Jasbir Singh, B. G.Mattias Luukkonen, Matt Lopper, Anton Bittner, Nancy E. Miller, Michael R. Jackson, Teresa Compton, Klaus Früh

Research output: Contribution to journalArticle

178 Scopus citations

Abstract

Human cytomegalovirus (HCMV) infection alters the expression of many cellular genes, including IFN-stimulated genes (ISGs) [Zhu, H., Cong, J.-P., Mamtora, G., Gingeras, T. & Shenk, T. (1998) Proc. Natl. Acad. Sci. USA 95, 14470-14475]. By using high-density cDNA microarrays, we show that the HCMV-regulated gene expression profile in fibroblasts does not differ substantially from the response generated by IFN. Furthermore, we identified the specific viral component triggering this response as the envelope glycoprotein B (gB). Cells treated with gB, but not other herpesviral glycoproteins, exhibited the same transcriptional profile as HCMV-infected cells. Thus, the interaction of gB with its as yet unidentified cellular receptor is the principal mechanism by which HCMV alters cellular gene expression early during infection. These findings highlight a pioneering paradigm for the consequences of virus-receptor interactions.

Original languageEnglish (US)
Pages (from-to)7140-7145
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number13
DOIs
StatePublished - Jun 19 2001

ASJC Scopus subject areas

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