Global analysis of salmonella alternative sigma factor e on protein translation

Jie Li, Ernesto S. Nakayasu, Christopher C. Overall, Rudd C. Johnson, Afshan S. Kidwai, Jason E. McDermott, Charles Ansong, Fred Heffron, Eric D. Cambronne, Joshua N. Adkins

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The alternative sigma factor E (σE) is critical for response to extracytoplasmic stress in Salmonella. Extensive studies have been conducted on σE-regulated gene expression, particularly at the transcriptional level. Increasing evidence suggests however that σE may indirectly participate in post-transcriptional regulation. In this study, we conducted sample-matched global proteomic and transcriptomic analyses to determine the level of regulation mediated by σE in Salmonella. Samples were analyzed from wild-type and isogenic rpoE mutant Salmonella cultivated in three different conditions: nutrient-rich and conditions that mimic early and late intracellular infection. We found that 30% of the observed proteome was regulated by σE combining all three conditions. In different growth conditions, σE affected the expression of a broad spectrum of Salmonella proteins required for miscellaneous functions. Those involved in transport and binding, protein synthesis, and stress response were particularly highlighted. By comparing transcriptomic and proteomic data, we identified genes post-transcriptionally regulated by σE and found that post-transcriptional regulation was responsible for a majority of changes observed in the σE-regulated proteome. Further, comparison of transcriptomic and proteomic data from hfq mutant of Salmonella demonstrated that σE-mediated post-transcriptional regulation was partially dependent on the RNA-binding protein Hfq.

Original languageEnglish (US)
Pages (from-to)1716-1726
Number of pages11
JournalJournal of Proteome Research
Volume14
Issue number4
DOIs
StatePublished - Apr 3 2015

Keywords

  • Salmonella
  • infection
  • post-transcriptional regulation
  • proteomics
  • sigma factor E
  • transcriptomics
  • virulence

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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