Glioma-associated endothelial cells show evidence of replicative senescence

Christiana Charalambous, Jenilyn Virrey, Adel Kardosh, Mark N. Jabbour, Lubna Qazi-Abdullah, Ligaya Pen, Raphael Zidovetzki, Axel H. Schönthal, Thomas C. Chen, Florence M. Hofman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The innately programmed process of replicative senescence has been studied extensively with respect to cancer, but primarily from the perspective of tumor cells overcoming this stringent innate barrier and acquiring the capacity for unlimited proliferation. In this study, we focus on the potential role of replicative senescence affecting the non-transformed endothelial cells of the blood vessels within the tumor microenvironment. Based on the well-documented aberrant structural and functional features of blood vessels within solid tumors, we hypothesized that tumor-derived factors may lead to premature replicative senescence in tumor-associated brain endothelial cells (TuBEC). We show here that glioma tissue, but not normal brain tissue, contains cells that express the signature of replicative senescence, senescence-associated β-galactosidase (SA-β-gal), on CD31-positive endothelial cells. Primary cultures of human TuBEC stain for SA-β-gal and exhibit characteristics of replicative senescence, including increased levels of the cell cycle inhibitors p21 and p27, increased resistance to cytotoxic drugs, increased growth factor production, and inability to proliferate. These data provide the first demonstration that tumor-derived brain endothelial cells may have reached an end-stage of differentiation known as replicative senescence and underscore the need for anti-angiogenic therapies to target this unique tumor-associated endothelial cell population.

Original languageEnglish (US)
Pages (from-to)1192-1202
Number of pages11
JournalExperimental Cell Research
Volume313
Issue number6
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

Fingerprint

Cell Aging
Glioma
Endothelial Cells
Galactosidases
Brain Neoplasms
Neoplasms
Vascular Tissue Neoplasms
Tumor Microenvironment
Blood Vessels
Intercellular Signaling Peptides and Proteins
Cell Cycle
Coloring Agents
Brain
Pharmaceutical Preparations
Population

Keywords

  • Endothelial cells
  • Glioblastoma
  • Microvasculature
  • Senescence
  • Tumor

ASJC Scopus subject areas

  • Cell Biology

Cite this

Charalambous, C., Virrey, J., Kardosh, A., Jabbour, M. N., Qazi-Abdullah, L., Pen, L., ... Hofman, F. M. (2007). Glioma-associated endothelial cells show evidence of replicative senescence. Experimental Cell Research, 313(6), 1192-1202. https://doi.org/10.1016/j.yexcr.2006.12.027

Glioma-associated endothelial cells show evidence of replicative senescence. / Charalambous, Christiana; Virrey, Jenilyn; Kardosh, Adel; Jabbour, Mark N.; Qazi-Abdullah, Lubna; Pen, Ligaya; Zidovetzki, Raphael; Schönthal, Axel H.; Chen, Thomas C.; Hofman, Florence M.

In: Experimental Cell Research, Vol. 313, No. 6, 01.04.2007, p. 1192-1202.

Research output: Contribution to journalArticle

Charalambous, C, Virrey, J, Kardosh, A, Jabbour, MN, Qazi-Abdullah, L, Pen, L, Zidovetzki, R, Schönthal, AH, Chen, TC & Hofman, FM 2007, 'Glioma-associated endothelial cells show evidence of replicative senescence', Experimental Cell Research, vol. 313, no. 6, pp. 1192-1202. https://doi.org/10.1016/j.yexcr.2006.12.027
Charalambous, Christiana ; Virrey, Jenilyn ; Kardosh, Adel ; Jabbour, Mark N. ; Qazi-Abdullah, Lubna ; Pen, Ligaya ; Zidovetzki, Raphael ; Schönthal, Axel H. ; Chen, Thomas C. ; Hofman, Florence M. / Glioma-associated endothelial cells show evidence of replicative senescence. In: Experimental Cell Research. 2007 ; Vol. 313, No. 6. pp. 1192-1202.
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