Glial wingless/wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction

Kimberly S. Kerr, Yuly Fuentes-Medel, Cassandra Brewer, Romina Barria, James Ashley, Katharine C. Abruzzi, Amy Sheehan, Ozge E. Tasdemir-Yilmaz, Marc Freeman, Vivian Budnik

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Glial cells are emerging as important regulators of synapse formation, maturation, and plasticity through the release of secreted signaling molecules. Here we use chromatin immunoprecipitation along with Drosophila genomic tiling arrays to define potential targets of the glial transcription factor Reversed polarity (Repo). Unexpectedly, we identified wingless (wg), a secreted morphogen that regulates synaptic growth at the Drosophila larval neuromuscular junction (NMJ), as a potential Repo target gene. We demonstrate that Repo regulates wg expression in vivo and that local glial cells secrete Wg at the NMJ to regulate glutamate receptor clustering and synaptic function. This work identifies Wg as a novel in vivo glial-secreted factor that specifically modulates assembly of the postsynaptic signaling machinery at the Drosophila NMJ.

Original languageEnglish (US)
Pages (from-to)2910-2920
Number of pages11
JournalJournal of Neuroscience
Volume34
Issue number8
DOIs
StatePublished - 2014
Externally publishedYes

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Neuromuscular Junction
Glutamate Receptors
Neuroglia
Drosophila
Cluster Analysis
Chromatin Immunoprecipitation
Synapses
Transcription Factors
Growth
Genes

Keywords

  • Drosophila
  • Glia
  • NMJ
  • Synapse
  • Wnt/Wg

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kerr, K. S., Fuentes-Medel, Y., Brewer, C., Barria, R., Ashley, J., Abruzzi, K. C., ... Budnik, V. (2014). Glial wingless/wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction. Journal of Neuroscience, 34(8), 2910-2920. https://doi.org/10.1523/JNEUROSCI.3714-13.2014

Glial wingless/wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction. / Kerr, Kimberly S.; Fuentes-Medel, Yuly; Brewer, Cassandra; Barria, Romina; Ashley, James; Abruzzi, Katharine C.; Sheehan, Amy; Tasdemir-Yilmaz, Ozge E.; Freeman, Marc; Budnik, Vivian.

In: Journal of Neuroscience, Vol. 34, No. 8, 2014, p. 2910-2920.

Research output: Contribution to journalArticle

Kerr, KS, Fuentes-Medel, Y, Brewer, C, Barria, R, Ashley, J, Abruzzi, KC, Sheehan, A, Tasdemir-Yilmaz, OE, Freeman, M & Budnik, V 2014, 'Glial wingless/wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction', Journal of Neuroscience, vol. 34, no. 8, pp. 2910-2920. https://doi.org/10.1523/JNEUROSCI.3714-13.2014
Kerr, Kimberly S. ; Fuentes-Medel, Yuly ; Brewer, Cassandra ; Barria, Romina ; Ashley, James ; Abruzzi, Katharine C. ; Sheehan, Amy ; Tasdemir-Yilmaz, Ozge E. ; Freeman, Marc ; Budnik, Vivian. / Glial wingless/wnt regulates glutamate receptor clustering and synaptic physiology at the Drosophila neuromuscular junction. In: Journal of Neuroscience. 2014 ; Vol. 34, No. 8. pp. 2910-2920.
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