Gestational hyperglycemia reprograms cardiac gene expression in rat offspring

Lara Lehtoranta, Anna Koskinen, Olli Vuolteenaho, Jukka Laine, Ville Kytö, Hanna Soukka, Eeva Ekholm, Juha Rasanen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

BackgroundRat fetuses with maternal pregestational hyperglycemia develop cardiac dysfunction, and their cardiac gene expression differs from that of healthy control fetuses near term. We hypothesized that cardiac gene expression and morphologic abnormalities of rat fetuses with maternal pregestational hyperglycemia become normal after birth.MethodsNine rats were preconceptually injected with streptozotocin to induce maternal hyperglycemia and nine rats served as controls. The hyperglycemia group comprised 82 mice and the control group 74 offspring fed by euglycemic dams. Hearts of the offspring were collected on postnatal days 0, 7, and 14, and processed for histologic and gene expression analyses.ResultsOn day 0, heart weight was increased, and expression of cardiac genes involved in contractility, growth, and metabolism was decreased in the hyperglycemia group. On day 7, although cardiomyocyte apoptosis was enhanced, most of the changes in gene expression had normalized in the hyperglycemia group. By day 14, the expression of genes important for myocardial growth, function, and metabolism was again abnormal in the hyperglycemia group.ConclusionMost cardiac gene expression abnormalities become transiently normal during the first week of life of offspring to hyperglycemic rats. However, by day 14, cardiac expressions of genes involved in growth, function, and metabolism are again abnormal in relation to control offspring.

Original languageEnglish (US)
Pages (from-to)356-361
Number of pages6
JournalPediatric Research
Volume82
Issue number2
DOIs
StatePublished - Aug 1 2017
Externally publishedYes

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Hyperglycemia
Gene Expression
Fetus
Mothers
Growth
Pseudogenes
Streptozocin
Cardiac Myocytes
Parturition
Apoptosis
Weights and Measures
Control Groups

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Lehtoranta, L., Koskinen, A., Vuolteenaho, O., Laine, J., Kytö, V., Soukka, H., ... Rasanen, J. (2017). Gestational hyperglycemia reprograms cardiac gene expression in rat offspring. Pediatric Research, 82(2), 356-361. https://doi.org/10.1038/pr.2017.42

Gestational hyperglycemia reprograms cardiac gene expression in rat offspring. / Lehtoranta, Lara; Koskinen, Anna; Vuolteenaho, Olli; Laine, Jukka; Kytö, Ville; Soukka, Hanna; Ekholm, Eeva; Rasanen, Juha.

In: Pediatric Research, Vol. 82, No. 2, 01.08.2017, p. 356-361.

Research output: Contribution to journalArticle

Lehtoranta, L, Koskinen, A, Vuolteenaho, O, Laine, J, Kytö, V, Soukka, H, Ekholm, E & Rasanen, J 2017, 'Gestational hyperglycemia reprograms cardiac gene expression in rat offspring', Pediatric Research, vol. 82, no. 2, pp. 356-361. https://doi.org/10.1038/pr.2017.42
Lehtoranta L, Koskinen A, Vuolteenaho O, Laine J, Kytö V, Soukka H et al. Gestational hyperglycemia reprograms cardiac gene expression in rat offspring. Pediatric Research. 2017 Aug 1;82(2):356-361. https://doi.org/10.1038/pr.2017.42
Lehtoranta, Lara ; Koskinen, Anna ; Vuolteenaho, Olli ; Laine, Jukka ; Kytö, Ville ; Soukka, Hanna ; Ekholm, Eeva ; Rasanen, Juha. / Gestational hyperglycemia reprograms cardiac gene expression in rat offspring. In: Pediatric Research. 2017 ; Vol. 82, No. 2. pp. 356-361.
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