Genotype analysis of polymorphisms in autoimmune susceptibility genes, CTLA-4 and PTPN22, in an Acute anterior uveitis cohort

Tammy Martin, Louise Bye, Neil Modi, Miles R. Stanford, Robert Vaughan, Justine R. Smith, N. Kevin Wade, Friederike Mackensen, Eric Suhler, James (Jim) Rosenbaum, Graham R. Wallace

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: Acute anterior uveitis (AAU) is the most common form of uveitis and is thought to be autoimmune in nature. Recent studies have described genes that act as master controllers of autoimmunity. Protein tyrosine phosphatase type 22 (PTPN22) and Cytotoxic T lymphocyte antigen-4 (CTLA-4) are two of these genes, and single nucleotide polymorphisms (SNPs) in the genes encoding these molecules have been associated with several autoimmune diseases. In this study we have analyzed SNPs in PTPN22 and CTLA-4 in patients with AAU. Methods: The functional protein tyrosine phosphatase type 22 (PTPN22) SNP (R620W rs2476601, 1858C/T), and two CTLA-4 SNPs (rs5742909, -318C/T and rs231775, 49A/G) were analyzed in 140 patients with AAU and 92 healthy controls by sequence-specific primer -polymerase chain reaction (SSP-PCR). Data was analyzed by χ2 analysis and Fisher's exact test. Results: There was no significant association between PTPN22 620W, CTLA-4 -318C/T, or CTLA-4 49A/G and AAU. Similarly, there was no association with the three SNPs when patients were classified by race or gender. Finally, there was no association with the presence of ankylosing spondylitis in the patient cohort. Conclusions: The data do not support an association between SNPs in PTPN22 and CTLA-4, genes regarded as genetic master switches of autoimmunity. This raises the issue of the etiology of AAU and the possibility that it should be regarded as an autoinflammatory rather than an autoimmune condition.

Original languageEnglish (US)
Pages (from-to)208-212
Number of pages5
JournalMolecular Vision
Volume15
StatePublished - Jan 26 2009

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CTLA-4 Antigen
Anterior Uveitis
Single Nucleotide Polymorphism
Genotype
Genes
Protein Tyrosine Phosphatases
Autoimmunity
Ankylosing Spondylitis
Uveitis
Autoimmune Diseases
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Martin, T., Bye, L., Modi, N., Stanford, M. R., Vaughan, R., Smith, J. R., ... Wallace, G. R. (2009). Genotype analysis of polymorphisms in autoimmune susceptibility genes, CTLA-4 and PTPN22, in an Acute anterior uveitis cohort. Molecular Vision, 15, 208-212.

Genotype analysis of polymorphisms in autoimmune susceptibility genes, CTLA-4 and PTPN22, in an Acute anterior uveitis cohort. / Martin, Tammy; Bye, Louise; Modi, Neil; Stanford, Miles R.; Vaughan, Robert; Smith, Justine R.; Wade, N. Kevin; Mackensen, Friederike; Suhler, Eric; Rosenbaum, James (Jim); Wallace, Graham R.

In: Molecular Vision, Vol. 15, 26.01.2009, p. 208-212.

Research output: Contribution to journalArticle

Martin, T, Bye, L, Modi, N, Stanford, MR, Vaughan, R, Smith, JR, Wade, NK, Mackensen, F, Suhler, E, Rosenbaum, JJ & Wallace, GR 2009, 'Genotype analysis of polymorphisms in autoimmune susceptibility genes, CTLA-4 and PTPN22, in an Acute anterior uveitis cohort', Molecular Vision, vol. 15, pp. 208-212.
Martin, Tammy ; Bye, Louise ; Modi, Neil ; Stanford, Miles R. ; Vaughan, Robert ; Smith, Justine R. ; Wade, N. Kevin ; Mackensen, Friederike ; Suhler, Eric ; Rosenbaum, James (Jim) ; Wallace, Graham R. / Genotype analysis of polymorphisms in autoimmune susceptibility genes, CTLA-4 and PTPN22, in an Acute anterior uveitis cohort. In: Molecular Vision. 2009 ; Vol. 15. pp. 208-212.
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AU - Modi, Neil

AU - Stanford, Miles R.

AU - Vaughan, Robert

AU - Smith, Justine R.

AU - Wade, N. Kevin

AU - Mackensen, Friederike

AU - Suhler, Eric

AU - Rosenbaum, James (Jim)

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N2 - Purpose: Acute anterior uveitis (AAU) is the most common form of uveitis and is thought to be autoimmune in nature. Recent studies have described genes that act as master controllers of autoimmunity. Protein tyrosine phosphatase type 22 (PTPN22) and Cytotoxic T lymphocyte antigen-4 (CTLA-4) are two of these genes, and single nucleotide polymorphisms (SNPs) in the genes encoding these molecules have been associated with several autoimmune diseases. In this study we have analyzed SNPs in PTPN22 and CTLA-4 in patients with AAU. Methods: The functional protein tyrosine phosphatase type 22 (PTPN22) SNP (R620W rs2476601, 1858C/T), and two CTLA-4 SNPs (rs5742909, -318C/T and rs231775, 49A/G) were analyzed in 140 patients with AAU and 92 healthy controls by sequence-specific primer -polymerase chain reaction (SSP-PCR). Data was analyzed by χ2 analysis and Fisher's exact test. Results: There was no significant association between PTPN22 620W, CTLA-4 -318C/T, or CTLA-4 49A/G and AAU. Similarly, there was no association with the three SNPs when patients were classified by race or gender. Finally, there was no association with the presence of ankylosing spondylitis in the patient cohort. Conclusions: The data do not support an association between SNPs in PTPN22 and CTLA-4, genes regarded as genetic master switches of autoimmunity. This raises the issue of the etiology of AAU and the possibility that it should be regarded as an autoinflammatory rather than an autoimmune condition.

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