TY - JOUR
T1 - Genomic organization of rat rDNA
AU - Chikaraishi, Dona M.
AU - Buchanan, Lisa
AU - Danna, Kathleen J.
AU - Harrington, Christina A.
N1 - Funding Information:
This work was supported by NIH Grants GM29302 and GM29986 to D.M.C. and NIH Grant CA18212 to K.J.D. C.A.H. was supported by NIH Predoctoral Training Grant 5T32 GM 07385-04 from 1/81 to 7/81.
PY - 1983/9/24
Y1 - 1983/9/24
N2 - A detailed restriction map was determined for a 10.9 KB region that contains the initiation site for 45S pre-rRNA and the first 1.7 KB of the 18S rRNA coding region. When the restriction pattern of the cloned rDNA was compared with that of total rat DNA, ther DNA regions of both Sprague-Dawley and BD-9 rats were identical to each other and to that of the cloned rDNA. However, both strains exhibit a major polymorphism consisting of an insertion of 0.9KB of DNA in the nontranscribed space rbetween 0.29 KB and 1.8 KB upstream from the 45S RNA initiation site.This region consists of tandem repeats approximately 130 base pairs in length. These repeats contain large poly T tracts and are similar in sequence to analogous elements 5′ to the origin of mouse rRNA transcription. Regions containing highly repetitious DNA sequences were located at sites 2.8 KB and 4.3 KB upstream from the initiation site. The repetitive sequence at 2.8 KB from the initiation site anneal to a known Alu-equivalent type 2 sequence derived from the second intron of the rat growth hormone gene.
AB - A detailed restriction map was determined for a 10.9 KB region that contains the initiation site for 45S pre-rRNA and the first 1.7 KB of the 18S rRNA coding region. When the restriction pattern of the cloned rDNA was compared with that of total rat DNA, ther DNA regions of both Sprague-Dawley and BD-9 rats were identical to each other and to that of the cloned rDNA. However, both strains exhibit a major polymorphism consisting of an insertion of 0.9KB of DNA in the nontranscribed space rbetween 0.29 KB and 1.8 KB upstream from the 45S RNA initiation site.This region consists of tandem repeats approximately 130 base pairs in length. These repeats contain large poly T tracts and are similar in sequence to analogous elements 5′ to the origin of mouse rRNA transcription. Regions containing highly repetitious DNA sequences were located at sites 2.8 KB and 4.3 KB upstream from the initiation site. The repetitive sequence at 2.8 KB from the initiation site anneal to a known Alu-equivalent type 2 sequence derived from the second intron of the rat growth hormone gene.
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U2 - 10.1093/nar/11.18.6437
DO - 10.1093/nar/11.18.6437
M3 - Article
C2 - 6312424
AN - SCOPUS:0021111304
SN - 0305-1048
VL - 11
SP - 6437
EP - 6452
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 18
ER -