Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila

Lawrence Salkoff, Alice Butler, Aguan Wei, Nancy Scavarda, Kelleen Giffen, Catherine Ifune, Richard Goodman, Gail Mandel

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

The deduced amino acid sequence of a Drosophila gene isolated with a vertebrate sodium channel complementary DNA probe revealed an organization virtually identical to the vertebrate sodium channel protein; four homologous domains containing all putative membrane-spanning regions are repeated in tandem with connecting linkers of various sizes. All areas of the protein presumed to be critical for channel function show high evolutionary conservation. These include those proposed to function in voltage-sensitive gating, inactivation, and ion selectivity. All 24 putative gating charges of the vertebrate protein are in identical positions in the Drosophila gene. Ten introns interrupt the coding regions of the four homology units; introns with positions conserved among homology units bracket a region hypothesized to be the selectivity filter for the channel. The Drosophila gene maps to the right arm of the second chromosome in region 60D-E. This position does not coincide with any known mutations that confer behavioral phenotypes, but is close to the seizure locus (60A-B), which has been hypothesized to code for a voltage-sensitive sodium channel.

Original languageEnglish (US)
Pages (from-to)744-749
Number of pages6
JournalScience
Volume237
Issue number4816
StatePublished - 1987
Externally publishedYes

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Sodium Channels
Drosophila
Vertebrates
Amino Acid Sequence
Introns
Genes
Proteins
DNA Probes
Seizures
Complementary DNA
Chromosomes
Ions
Phenotype
Mutation
Membranes

ASJC Scopus subject areas

  • General

Cite this

Salkoff, L., Butler, A., Wei, A., Scavarda, N., Giffen, K., Ifune, C., ... Mandel, G. (1987). Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila. Science, 237(4816), 744-749.

Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila. / Salkoff, Lawrence; Butler, Alice; Wei, Aguan; Scavarda, Nancy; Giffen, Kelleen; Ifune, Catherine; Goodman, Richard; Mandel, Gail.

In: Science, Vol. 237, No. 4816, 1987, p. 744-749.

Research output: Contribution to journalArticle

Salkoff, L, Butler, A, Wei, A, Scavarda, N, Giffen, K, Ifune, C, Goodman, R & Mandel, G 1987, 'Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila', Science, vol. 237, no. 4816, pp. 744-749.
Salkoff L, Butler A, Wei A, Scavarda N, Giffen K, Ifune C et al. Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila. Science. 1987;237(4816):744-749.
Salkoff, Lawrence ; Butler, Alice ; Wei, Aguan ; Scavarda, Nancy ; Giffen, Kelleen ; Ifune, Catherine ; Goodman, Richard ; Mandel, Gail. / Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila. In: Science. 1987 ; Vol. 237, No. 4816. pp. 744-749.
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AU - Butler, Alice

AU - Wei, Aguan

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AU - Giffen, Kelleen

AU - Ifune, Catherine

AU - Goodman, Richard

AU - Mandel, Gail

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