Genomic island TnSmu2 of Streptococcus mutans harbors a nonribosomal peptide synthetase-polyketide synthase gene cluster responsible for the biosynthesis of pigments involved in oxygen and H2O2 tolerance

Chenggang Wu, Robert Cichewicz, Yihong Li, Jinman Liu, Bruce Roe, Joseph Ferretti, Justin Merritt, Fengxia Qi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The oral biofilm community consists of >800 microbial species, among which Streptococcus mutans is considered a primary pathogen for dental caries. The genomic island TnSmu2 of S. mutans comprises >2% of the genome. In this study, we demonstrate that TnSmu2 harbors a gene cluster encoding nonribosomal peptide synthetases (NRPS), polyketide synthases (PKS), and accessory proteins and regulators involved in nonribosomal peptide (NRP) and polyketide (PK) biosynthesis. Interestingly, the sequences of these genes and their genomic organizations and locations are highly divergent among different S. mutans strains, yet each TnSmu2 region encodes NRPS/PKS and accessory proteins. Mutagenesis of the structural genes and putative regulatory genes in strains UA159, UA140, and MT4653 resulted in colonies that were devoid of their yellow pigmentation (for strains UA140 and MT4653). In addition, these mutant strains also displayed retarded growth under aerobic conditions and in the presence of H2O2. High-performance liquid chromatography profiling of cell surface extracts identified unique peaks that were missing in the mutant strains, and partial characterization of the purified product from UA159 demonstrated that it is indeed a hybrid NRP/PK, as predicted. A genomic survey of 94 clinical S. mutans isolates suggests that the TnSmu2 gene cluster may be more prevalent than previously recognized.

Original languageEnglish (US)
Pages (from-to)5815-5826
Number of pages12
JournalApplied and Environmental Microbiology
Volume76
Issue number17
DOIs
StatePublished - Sep 2010
Externally publishedYes

Fingerprint

Peptide Synthases
Polyketide Synthases
genomic islands
polyketide synthases
Streptococcus mutans
Genomic Islands
Multigene Family
ligases
multigene family
peptide
pigment
genomics
harbor
tolerance
pigments
biosynthesis
peptides
Oxygen
Polyketides
oxygen

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Genomic island TnSmu2 of Streptococcus mutans harbors a nonribosomal peptide synthetase-polyketide synthase gene cluster responsible for the biosynthesis of pigments involved in oxygen and H2O2 tolerance. / Wu, Chenggang; Cichewicz, Robert; Li, Yihong; Liu, Jinman; Roe, Bruce; Ferretti, Joseph; Merritt, Justin; Qi, Fengxia.

In: Applied and Environmental Microbiology, Vol. 76, No. 17, 09.2010, p. 5815-5826.

Research output: Contribution to journalArticle

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