Genomic expression programs in the response of yeast cells to environmental changes

A. P. Gasch, Paul Spellman, C. M. Kao, O. Carmel-Harel, M. B. Eisen, G. Storz, D. Botstein, P. O. Brown

Research output: Contribution to journalArticle

3335 Citations (Scopus)

Abstract

We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. A large set of genes (~ 900) showed a similar drastic response to almost all of these environmental changes. Additional features of the genomic responses were specialized for specific conditions. Promoter analysis and subsequent characterization of the responses of mutant strains implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators. Physiological themes in the genomic responses to specific environmental stresses provided insights into the effects of those stresses on the cell.

Original languageEnglish (US)
Pages (from-to)4241-4257
Number of pages17
JournalMolecular Biology of the Cell
Volume11
Issue number12
StatePublished - 2000
Externally publishedYes

Fingerprint

Yeasts
Diamide
Vitamin K 3
Dithiothreitol
Reducing Agents
Osmotic Pressure
Starvation
Oligonucleotide Array Sequence Analysis
Oxidants
Superoxides
Disulfides
Hydrogen Peroxide
Genes
Saccharomyces cerevisiae
Shock
Transcription Factors
Nitrogen
Amino Acids
Temperature
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Gasch, A. P., Spellman, P., Kao, C. M., Carmel-Harel, O., Eisen, M. B., Storz, G., ... Brown, P. O. (2000). Genomic expression programs in the response of yeast cells to environmental changes. Molecular Biology of the Cell, 11(12), 4241-4257.

Genomic expression programs in the response of yeast cells to environmental changes. / Gasch, A. P.; Spellman, Paul; Kao, C. M.; Carmel-Harel, O.; Eisen, M. B.; Storz, G.; Botstein, D.; Brown, P. O.

In: Molecular Biology of the Cell, Vol. 11, No. 12, 2000, p. 4241-4257.

Research output: Contribution to journalArticle

Gasch, AP, Spellman, P, Kao, CM, Carmel-Harel, O, Eisen, MB, Storz, G, Botstein, D & Brown, PO 2000, 'Genomic expression programs in the response of yeast cells to environmental changes', Molecular Biology of the Cell, vol. 11, no. 12, pp. 4241-4257.
Gasch AP, Spellman P, Kao CM, Carmel-Harel O, Eisen MB, Storz G et al. Genomic expression programs in the response of yeast cells to environmental changes. Molecular Biology of the Cell. 2000;11(12):4241-4257.
Gasch, A. P. ; Spellman, Paul ; Kao, C. M. ; Carmel-Harel, O. ; Eisen, M. B. ; Storz, G. ; Botstein, D. ; Brown, P. O. / Genomic expression programs in the response of yeast cells to environmental changes. In: Molecular Biology of the Cell. 2000 ; Vol. 11, No. 12. pp. 4241-4257.
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