Genome-wide screening of potential RNase Y-processed mRNAs in the M49 serotype Streptococcus pyogenes NZ131

Zhiyun Chen, Rahul Raghavan, Fengxia Qi, Justin Merritt, Jens Kreth

Research output: Contribution to journalArticle

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Abstract

RNase Y is a major endoribonuclease in Group A streptococcus (GAS) and other Gram-positive bacteria. Our previous study showed that RNase Y was involved in mRNA degradation and processing in GAS. We hypothesized that mRNA processing regulated the expression of important GAS virulence factors via altering their mRNA stabilities and that RNase Y mediated at least some of the mRNA-processing events. The aims of this study were to (1) identify mRNAs that were processed by RNase Y and (2) confirm the mRNA-processing events. The transcriptomes of Streptococcus pyogenes NZ131 wild type and its RNase Y mutant (Δrny) were examined with RNA-seq. The data were further analyzed to define GAS operons. The mRNA stabilities of the wild type and Δrny at subgene level were determined with tiling array analysis. Operons displaying segmental stability in the wild type but not in the Δrny were predicted to be RNase Y processed. Overall 865 operons were defined and their boundaries predicted. Further analysis narrowed down 15 mRNAs potentially processed by RNase Y. A selection of four candidates including folC1 (folylpolyglutamate synthetase), prtF (fibronectin-binding protein), speG (streptococcal exotoxin G), ropB (transcriptional regulator of speB), and ypaA (riboflavin transporter) mRNAs was examined with Northern blot analysis. However, only folC1 was confirmed to be processed, but it is unlikely that RNase Y is responsible. We conclude that GAS use RNase Y to selectively process mRNA, but the overall impact is confined to selected virulence factors.

Original languageEnglish (US)
Article numbere00671
JournalMicrobiologyOpen
Volume8
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Streptococcus pyogenes
Ribonucleases
Genome
Messenger RNA
Streptococcus
RNA Stability
Operon
Virulence Factors
Endoribonucleases
Serogroup
Exotoxins
Riboflavin
Gram-Positive Bacteria
Transcriptome
Fibronectins
Northern Blotting
Carrier Proteins
RNA

Keywords

  • genomics
  • RNA processing
  • RNase Y
  • Streptococcus pyogenes

ASJC Scopus subject areas

  • Microbiology

Cite this

Genome-wide screening of potential RNase Y-processed mRNAs in the M49 serotype Streptococcus pyogenes NZ131. / Chen, Zhiyun; Raghavan, Rahul; Qi, Fengxia; Merritt, Justin; Kreth, Jens.

In: MicrobiologyOpen, Vol. 8, No. 4, e00671, 01.04.2019.

Research output: Contribution to journalArticle

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