Genome sequencing of SHH medulloblastoma predicts genotype-related response to smoothened inhibition

Marcel Kool, David T.W. Jones, Natalie Jäger, Paul A. Northcott, Trevor J. Pugh, Volker Hovestadt, Rosario M. Piro, L. Adriana Esparza, Shirley L. Markant, Marc Remke, Till Milde, Franck Bourdeaut, Marina Ryzhova, Dominik Sturm, Elke Pfaff, Sebastian Stark, Sonja Hutter, Huriye Sxeker-Cin, Pascal Johann, Sebastian Bender & 56 others Christin Schmidt, Tobias Rausch, David Shih, Jüri Reimand, Laura Sieber, Andrea Wittmann, Linda Linke, Hendrik Witt, Ursula D. Weber, Marc Zapatka, Rainer König, Rameen Beroukhim, Guillaume Bergthold, Peter Van Sluis, Richard Volckmann, Jan Koster, Rogier Versteeg, Sabine Schmidt, Stephan Wolf, Chris Lawerenz, Cynthia C. Bartholomae, Christof Von Kalle, Andreas Unterberg, Christel Herold-Mende, Silvia Hofer, Andreas E. Kulozik, Andreas Von Deimling, Wolfram Scheurlen, Jörg Felsberg, Guido Reifenberger, Martin Hasselblatt, John R. Crawford, Gerald A. Grant, Nada Jabado, Arie Perry, Cynthia Cowdrey, Sydney Croul, Gelareh Zadeh, Jan O. Korbel, Francois Doz, Olivier Delattre, Gary D. Bader, Martin G. McCabe, V. Peter Collins, Mark W. Kieran, Yoon-Jae Cho, Scott L. Pomeroy, Olaf Witt, Benedikt Brors, Michael D. Taylor, Ulrich Schüller, Andrey Korshunov, Roland Eils, Robert J. Wechsler-Reya, Peter Lichter, Stefan M. Pfister

Research output: Contribution to journalArticle

310 Citations (Scopus)

Abstract

Smoothened (SMO) inhibitors recently entered clinical trials for sonic-hedgehog-driven medulloblastoma (SHH-MB). Clinical response is highly variable. To understand the mechanism(s) of primary resistance and identify pathways cooperating with aberrant SHH signaling, we sequenced and profiled a large cohort of SHH-MBs (n= 133). SHH pathway mutations involved PTCH1 (across all age groups), SUFU (infants, including germline), and SMO (adults). Children >3 years old harbored an excess of downstream MYCN and GLI2 amplifications and frequent TP53 mutations, often in the germline, all of which were rare in infants and adults. Functional assays in different SHH-MB xenograft models demonstrated that SHH-MBs harboring a PTCH1 mutation were responsive to SMO inhibition, whereas tumors harboring an SUFU mutation or MYCN amplification were primarily resistant.

Original languageEnglish (US)
Pages (from-to)393-405
Number of pages13
JournalCancer Cell
Volume25
Issue number3
DOIs
StatePublished - Mar 17 2014
Externally publishedYes

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Medulloblastoma
Genotype
Genome
Mutation
Heterografts
Age Groups
Clinical Trials
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

Cite this

Kool, M., Jones, D. T. W., Jäger, N., Northcott, P. A., Pugh, T. J., Hovestadt, V., ... Pfister, S. M. (2014). Genome sequencing of SHH medulloblastoma predicts genotype-related response to smoothened inhibition. Cancer Cell, 25(3), 393-405. https://doi.org/10.1016/j.ccr.2014.02.004

Genome sequencing of SHH medulloblastoma predicts genotype-related response to smoothened inhibition. / Kool, Marcel; Jones, David T.W.; Jäger, Natalie; Northcott, Paul A.; Pugh, Trevor J.; Hovestadt, Volker; Piro, Rosario M.; Esparza, L. Adriana; Markant, Shirley L.; Remke, Marc; Milde, Till; Bourdeaut, Franck; Ryzhova, Marina; Sturm, Dominik; Pfaff, Elke; Stark, Sebastian; Hutter, Sonja; Sxeker-Cin, Huriye; Johann, Pascal; Bender, Sebastian; Schmidt, Christin; Rausch, Tobias; Shih, David; Reimand, Jüri; Sieber, Laura; Wittmann, Andrea; Linke, Linda; Witt, Hendrik; Weber, Ursula D.; Zapatka, Marc; König, Rainer; Beroukhim, Rameen; Bergthold, Guillaume; Van Sluis, Peter; Volckmann, Richard; Koster, Jan; Versteeg, Rogier; Schmidt, Sabine; Wolf, Stephan; Lawerenz, Chris; Bartholomae, Cynthia C.; Von Kalle, Christof; Unterberg, Andreas; Herold-Mende, Christel; Hofer, Silvia; Kulozik, Andreas E.; Von Deimling, Andreas; Scheurlen, Wolfram; Felsberg, Jörg; Reifenberger, Guido; Hasselblatt, Martin; Crawford, John R.; Grant, Gerald A.; Jabado, Nada; Perry, Arie; Cowdrey, Cynthia; Croul, Sydney; Zadeh, Gelareh; Korbel, Jan O.; Doz, Francois; Delattre, Olivier; Bader, Gary D.; McCabe, Martin G.; Collins, V. Peter; Kieran, Mark W.; Cho, Yoon-Jae; Pomeroy, Scott L.; Witt, Olaf; Brors, Benedikt; Taylor, Michael D.; Schüller, Ulrich; Korshunov, Andrey; Eils, Roland; Wechsler-Reya, Robert J.; Lichter, Peter; Pfister, Stefan M.

In: Cancer Cell, Vol. 25, No. 3, 17.03.2014, p. 393-405.

Research output: Contribution to journalArticle

Kool, M, Jones, DTW, Jäger, N, Northcott, PA, Pugh, TJ, Hovestadt, V, Piro, RM, Esparza, LA, Markant, SL, Remke, M, Milde, T, Bourdeaut, F, Ryzhova, M, Sturm, D, Pfaff, E, Stark, S, Hutter, S, Sxeker-Cin, H, Johann, P, Bender, S, Schmidt, C, Rausch, T, Shih, D, Reimand, J, Sieber, L, Wittmann, A, Linke, L, Witt, H, Weber, UD, Zapatka, M, König, R, Beroukhim, R, Bergthold, G, Van Sluis, P, Volckmann, R, Koster, J, Versteeg, R, Schmidt, S, Wolf, S, Lawerenz, C, Bartholomae, CC, Von Kalle, C, Unterberg, A, Herold-Mende, C, Hofer, S, Kulozik, AE, Von Deimling, A, Scheurlen, W, Felsberg, J, Reifenberger, G, Hasselblatt, M, Crawford, JR, Grant, GA, Jabado, N, Perry, A, Cowdrey, C, Croul, S, Zadeh, G, Korbel, JO, Doz, F, Delattre, O, Bader, GD, McCabe, MG, Collins, VP, Kieran, MW, Cho, Y-J, Pomeroy, SL, Witt, O, Brors, B, Taylor, MD, Schüller, U, Korshunov, A, Eils, R, Wechsler-Reya, RJ, Lichter, P & Pfister, SM 2014, 'Genome sequencing of SHH medulloblastoma predicts genotype-related response to smoothened inhibition', Cancer Cell, vol. 25, no. 3, pp. 393-405. https://doi.org/10.1016/j.ccr.2014.02.004
Kool M, Jones DTW, Jäger N, Northcott PA, Pugh TJ, Hovestadt V et al. Genome sequencing of SHH medulloblastoma predicts genotype-related response to smoothened inhibition. Cancer Cell. 2014 Mar 17;25(3):393-405. https://doi.org/10.1016/j.ccr.2014.02.004
Kool, Marcel ; Jones, David T.W. ; Jäger, Natalie ; Northcott, Paul A. ; Pugh, Trevor J. ; Hovestadt, Volker ; Piro, Rosario M. ; Esparza, L. Adriana ; Markant, Shirley L. ; Remke, Marc ; Milde, Till ; Bourdeaut, Franck ; Ryzhova, Marina ; Sturm, Dominik ; Pfaff, Elke ; Stark, Sebastian ; Hutter, Sonja ; Sxeker-Cin, Huriye ; Johann, Pascal ; Bender, Sebastian ; Schmidt, Christin ; Rausch, Tobias ; Shih, David ; Reimand, Jüri ; Sieber, Laura ; Wittmann, Andrea ; Linke, Linda ; Witt, Hendrik ; Weber, Ursula D. ; Zapatka, Marc ; König, Rainer ; Beroukhim, Rameen ; Bergthold, Guillaume ; Van Sluis, Peter ; Volckmann, Richard ; Koster, Jan ; Versteeg, Rogier ; Schmidt, Sabine ; Wolf, Stephan ; Lawerenz, Chris ; Bartholomae, Cynthia C. ; Von Kalle, Christof ; Unterberg, Andreas ; Herold-Mende, Christel ; Hofer, Silvia ; Kulozik, Andreas E. ; Von Deimling, Andreas ; Scheurlen, Wolfram ; Felsberg, Jörg ; Reifenberger, Guido ; Hasselblatt, Martin ; Crawford, John R. ; Grant, Gerald A. ; Jabado, Nada ; Perry, Arie ; Cowdrey, Cynthia ; Croul, Sydney ; Zadeh, Gelareh ; Korbel, Jan O. ; Doz, Francois ; Delattre, Olivier ; Bader, Gary D. ; McCabe, Martin G. ; Collins, V. Peter ; Kieran, Mark W. ; Cho, Yoon-Jae ; Pomeroy, Scott L. ; Witt, Olaf ; Brors, Benedikt ; Taylor, Michael D. ; Schüller, Ulrich ; Korshunov, Andrey ; Eils, Roland ; Wechsler-Reya, Robert J. ; Lichter, Peter ; Pfister, Stefan M. / Genome sequencing of SHH medulloblastoma predicts genotype-related response to smoothened inhibition. In: Cancer Cell. 2014 ; Vol. 25, No. 3. pp. 393-405.
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AU - Korshunov, Andrey

AU - Eils, Roland

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AU - Lichter, Peter

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