Genetics identification of comS, a gene of the srfA operon that regulates the establishment of genetic competence in Bacillus subtilis

Cletus D'Souza, Michiko Nakano, Peter Zuber

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Genetic competence (the ability to internalize exogenous DNA) in Bacillus subtilis is dependent on a regulatory pathway that activates the expression of a battery of competence-specific genes. The srfA operon, encoding the subunits of surfactin synthetase, which catalyzes the nonribosomal synthesis of the peptide antibiotic surfactin, also functions in the competence regulatory pathway. The DNA encoding only one of the seven amino acid-activating domains of surfactin synthetase, the valine-activating domain (srfAB1), is necessary for competence. Deletion analysis revealed that a 569-bp fragment of srfAB1, fused to the srfA promoter, complements a srfA deletion mutation (Δ srfA) with respect to competence. This fragment contains an open reading frame consisting of 46 amino acids (orf46), which is out of frame with srfAB1. A frameshift mutation in srfAB upstream of orf46 has no effect on competence but a frameshift and nonsense mutation in orf46 resulted in failure to complement the ΔsrfA mutation. These results indicate that orf46 encodes the srfA-associated competence regulatory factor. Computer-aided analysis of the putative orf46 product (ComS) shows similarity to the homeodomain of the POJJ domain class of eukaryotic transcriptional regulators.

Original languageEnglish (US)
Pages (from-to)9397-9401
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number20
StatePublished - Sep 27 1994
Externally publishedYes

Fingerprint

Operon
Bacillus subtilis
Mental Competency
Genes
Frameshift Mutation
Amino Acids
Aptitude
Nonsense Codon
Sequence Deletion
DNA
Valine
Open Reading Frames
Anti-Bacterial Agents
Mutation

Keywords

  • Genetic competence
  • Peptide antibiotic biosynthesis
  • Transcriptional regulation

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "Genetic competence (the ability to internalize exogenous DNA) in Bacillus subtilis is dependent on a regulatory pathway that activates the expression of a battery of competence-specific genes. The srfA operon, encoding the subunits of surfactin synthetase, which catalyzes the nonribosomal synthesis of the peptide antibiotic surfactin, also functions in the competence regulatory pathway. The DNA encoding only one of the seven amino acid-activating domains of surfactin synthetase, the valine-activating domain (srfAB1), is necessary for competence. Deletion analysis revealed that a 569-bp fragment of srfAB1, fused to the srfA promoter, complements a srfA deletion mutation (Δ srfA) with respect to competence. This fragment contains an open reading frame consisting of 46 amino acids (orf46), which is out of frame with srfAB1. A frameshift mutation in srfAB upstream of orf46 has no effect on competence but a frameshift and nonsense mutation in orf46 resulted in failure to complement the ΔsrfA mutation. These results indicate that orf46 encodes the srfA-associated competence regulatory factor. Computer-aided analysis of the putative orf46 product (ComS) shows similarity to the homeodomain of the POJJ domain class of eukaryotic transcriptional regulators.",
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T1 - Genetics identification of comS, a gene of the srfA operon that regulates the establishment of genetic competence in Bacillus subtilis

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AU - Nakano, Michiko

AU - Zuber, Peter

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N2 - Genetic competence (the ability to internalize exogenous DNA) in Bacillus subtilis is dependent on a regulatory pathway that activates the expression of a battery of competence-specific genes. The srfA operon, encoding the subunits of surfactin synthetase, which catalyzes the nonribosomal synthesis of the peptide antibiotic surfactin, also functions in the competence regulatory pathway. The DNA encoding only one of the seven amino acid-activating domains of surfactin synthetase, the valine-activating domain (srfAB1), is necessary for competence. Deletion analysis revealed that a 569-bp fragment of srfAB1, fused to the srfA promoter, complements a srfA deletion mutation (Δ srfA) with respect to competence. This fragment contains an open reading frame consisting of 46 amino acids (orf46), which is out of frame with srfAB1. A frameshift mutation in srfAB upstream of orf46 has no effect on competence but a frameshift and nonsense mutation in orf46 resulted in failure to complement the ΔsrfA mutation. These results indicate that orf46 encodes the srfA-associated competence regulatory factor. Computer-aided analysis of the putative orf46 product (ComS) shows similarity to the homeodomain of the POJJ domain class of eukaryotic transcriptional regulators.

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