Genetically obese zucker rats have abnormally low brain insulin content

Denis G. Baskin; Leslie J. Stein; Hitoshi Ikeda; Stephen C. Woods; Dianne P. Figlewicz; Daniel Porte; M. R.C. Greenwood; Daniel M. Dorsa

doi:10.1016/0024-3205(85)90166-3

Genetically obese zucker rats have abnormally low brain insulin content

Denis G. Baskin, Leslie J. Stein, Hitoshi Ikeda, Stephen C. Woods, Dianne P. Figlewicz, Daniel Porte, M. R.C. Greenwood, Daniel M. Dorsa

Research output: Contribution to journal › Article › peer-review

126 Scopus citations

Abstract

The concentration of immunoreactive insulin (IRI) extracted from the olfactory bulb, hypothalamus, hippocampus, cerebral cortex, amygdala, midbrain, and hindbrain was significantly lower in obese (fa/fa) and heterozygous (Fa/fa) Zucker rats in comparison to lean (Fa/Fa) Zucker rats. This deficit in brain IRI content was most severe in the hypothalamus and olfactory bulb and was independent of severe obesity since the marked reduction of brain IRI content was also found in heterozygous rats which possessed only one copy of the fa allele. These results demonstrate that in the 2-3 month-old female Zucker rat, the fa allele is associated with defective regulation of insulin in the brain.

Original language	English (US)
Pages (from-to)	627-633
Number of pages	7
Journal	Life Sciences
Volume	36
Issue number	7
DOIs	https://doi.org/10.1016/0024-3205(85)90166-3
State	Published - Feb 18 1985
Externally published	Yes

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(85)90166-3

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@article{3687152ec7034a35b942bff395e92c9a,

title = "Genetically obese zucker rats have abnormally low brain insulin content",

abstract = "The concentration of immunoreactive insulin (IRI) extracted from the olfactory bulb, hypothalamus, hippocampus, cerebral cortex, amygdala, midbrain, and hindbrain was significantly lower in obese (fa/fa) and heterozygous (Fa/fa) Zucker rats in comparison to lean (Fa/Fa) Zucker rats. This deficit in brain IRI content was most severe in the hypothalamus and olfactory bulb and was independent of severe obesity since the marked reduction of brain IRI content was also found in heterozygous rats which possessed only one copy of the fa allele. These results demonstrate that in the 2-3 month-old female Zucker rat, the fa allele is associated with defective regulation of insulin in the brain.",

author = "Baskin, {Denis G.} and Stein, {Leslie J.} and Hitoshi Ikeda and Woods, {Stephen C.} and Figlewicz, {Dianne P.} and Daniel Porte and Greenwood, {M. R.C.} and Dorsa, {Daniel M.}",

note = "Funding Information: This research was supported by NIH Grant AM 17047 to the Diabetes Research Center, University of Washington, NIH Grants AM 12829, AA4 06~83, and AM 17844, and the Veterans Administration. Dr. Barbara McKnight, Biostatistical Core, Diabetes Research Center, performed the statistical analysis. Dr. Thomas Paquette, Radioimmunoassay Core, Diabetes F{esearch Center, prepared the trace insulin for the RIA. We thank Sharon Oliver-Clifner for expert technical assistance with the extractions and RIA, and Patricia Jenkins and kAaxine Cor nier for assistance with preparation of the manuscript.",

year = "1985",

month = feb,

day = "18",

doi = "10.1016/0024-3205(85)90166-3",

language = "English (US)",

volume = "36",

pages = "627--633",

journal = "Life Sciences",

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publisher = "Elsevier Inc.",

number = "7",

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T1 - Genetically obese zucker rats have abnormally low brain insulin content

AU - Baskin, Denis G.

AU - Stein, Leslie J.

AU - Ikeda, Hitoshi

AU - Woods, Stephen C.

AU - Figlewicz, Dianne P.

AU - Porte, Daniel

AU - Greenwood, M. R.C.

AU - Dorsa, Daniel M.

N1 - Funding Information: This research was supported by NIH Grant AM 17047 to the Diabetes Research Center, University of Washington, NIH Grants AM 12829, AA4 06~83, and AM 17844, and the Veterans Administration. Dr. Barbara McKnight, Biostatistical Core, Diabetes Research Center, performed the statistical analysis. Dr. Thomas Paquette, Radioimmunoassay Core, Diabetes F{esearch Center, prepared the trace insulin for the RIA. We thank Sharon Oliver-Clifner for expert technical assistance with the extractions and RIA, and Patricia Jenkins and kAaxine Cor nier for assistance with preparation of the manuscript.

PY - 1985/2/18

Y1 - 1985/2/18

N2 - The concentration of immunoreactive insulin (IRI) extracted from the olfactory bulb, hypothalamus, hippocampus, cerebral cortex, amygdala, midbrain, and hindbrain was significantly lower in obese (fa/fa) and heterozygous (Fa/fa) Zucker rats in comparison to lean (Fa/Fa) Zucker rats. This deficit in brain IRI content was most severe in the hypothalamus and olfactory bulb and was independent of severe obesity since the marked reduction of brain IRI content was also found in heterozygous rats which possessed only one copy of the fa allele. These results demonstrate that in the 2-3 month-old female Zucker rat, the fa allele is associated with defective regulation of insulin in the brain.

AB - The concentration of immunoreactive insulin (IRI) extracted from the olfactory bulb, hypothalamus, hippocampus, cerebral cortex, amygdala, midbrain, and hindbrain was significantly lower in obese (fa/fa) and heterozygous (Fa/fa) Zucker rats in comparison to lean (Fa/Fa) Zucker rats. This deficit in brain IRI content was most severe in the hypothalamus and olfactory bulb and was independent of severe obesity since the marked reduction of brain IRI content was also found in heterozygous rats which possessed only one copy of the fa allele. These results demonstrate that in the 2-3 month-old female Zucker rat, the fa allele is associated with defective regulation of insulin in the brain.

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VL - 36

SP - 627

EP - 633

JO - Life Sciences

JF - Life Sciences

IS - 7

ER -

Genetically obese zucker rats have abnormally low brain insulin content

Abstract

ASJC Scopus subject areas

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