Genetically encoded FRET probe for PKC activity based on pleckstrin

Andreas Schleifenbaum, Gunter Stier, Alexander Gasch, Michael Sattler, Garsten Schultz

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

We developed a probe for investigating protein kinase C (PKC) activity in living cells. The probe is based on a fragment of pleckstrin enclosed by two FRET-capable fluorophores. PKC activity modulation was reliably followed by FRET change in vitro and in vivo. The probe responds quickly to PKC activation by phorbol ester. FRET changes were reversible when PKC inhibitors were administered. Stimulation of cellular signaling pathways using histamine or bradykinin triggered PKC in a physiologically relevant way.

Original languageEnglish (US)
Pages (from-to)11786-11787
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number38
DOIs
StatePublished - Sep 29 2004

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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