Genetically encoded copper-free click chemistry

Tilman Plass, Sigrid Milles, Christine Koehler, Carsten Schultz, Edward A. Lemke

Research output: Contribution to journalArticlepeer-review

240 Scopus citations


A bright click of life: Strained alkynes are genetically encoded into E. coli by use of an engineered pyrrolysine amber suppressor tRNA/synthetase pair from Methanosarcina mazei. As a result cyclooctynyl lysine derivatives were incorporated site-specifically in proteins allowing efficient labeling with fluorogenic azide-bearing dyes by means of a click reaction under physiological conditions in vitro and in vivo (see scheme).

Original languageEnglish (US)
Pages (from-to)3878-3881
Number of pages4
JournalAngewandte Chemie - International Edition
Issue number17
StatePublished - Apr 18 2011
Externally publishedYes


  • amino acids
  • click chemistry
  • gene expression
  • proteins
  • strained molecules

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)


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