Genetically encoded copper-free click chemistry

Tilman Plass; Sigrid Milles; Christine Koehler; Carsten Schultz; Edward A. Lemke

doi:10.1002/anie.201008178

Genetically encoded copper-free click chemistry

Tilman Plass, Sigrid Milles, Christine Koehler, Carsten Schultz, Edward A. Lemke

Research output: Contribution to journal › Article › peer-review

240 Scopus citations

Abstract

A bright click of life: Strained alkynes are genetically encoded into E. coli by use of an engineered pyrrolysine amber suppressor tRNA/synthetase pair from Methanosarcina mazei. As a result cyclooctynyl lysine derivatives were incorporated site-specifically in proteins allowing efficient labeling with fluorogenic azide-bearing dyes by means of a click reaction under physiological conditions in vitro and in vivo (see scheme).

Original language	English (US)
Pages (from-to)	3878-3881
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	17
DOIs	https://doi.org/10.1002/anie.201008178
State	Published - Apr 18 2011
Externally published	Yes

Keywords

amino acids
click chemistry
gene expression
proteins
strained molecules

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201008178

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AU - Plass, Tilman

AU - Milles, Sigrid

AU - Koehler, Christine

AU - Schultz, Carsten

AU - Lemke, Edward A.

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KW - amino acids

KW - click chemistry

KW - gene expression

KW - proteins

KW - strained molecules

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Genetically encoded copper-free click chemistry

Abstract

Keywords

ASJC Scopus subject areas

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