Abstract
Veillonellae are one of the most prevalent and predominant microorganisms in both the supra- and subgingival plaques of the human oral cavity. Veillonellae's mutualistic relationships with the early, middle, and late colonizers of the oral cavity make them an important component of oral biofilm ecology. Unlike other ubiquitous early colonizers in the oral cavity, surprisingly little is known about Veillonella biology due to our lack of ability to genetically transform this group of bacteria. The objective of this study was to test the transformability of veillonellae. Using Veillonella parvula strain PK1910, we first obtained spontaneous mutations conferring streptomycin resistance. These mutations all carry a K43N substitution in the RpsL protein. Using the mutated rpsL gene as a selection marker, a variety of conditions were tested and optimized for electroporation. With the optimized protocol, we were able to introduce the first targeted mutation into the chromosome of V. parvula PK1910. Although more studies are needed to develop a robust genetic manipulation system in veillonellae, our results demonstrated, for the first time, that V. parvula is transformable, at least for strain PK1910.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 138-144 |
| Number of pages | 7 |
| Journal | FEMS Microbiology Letters |
| Volume | 322 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 2011 |
| Externally published | Yes |
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Keywords
- Mutagenesis
- Oral microbiology
- Veillonellae
ASJC Scopus subject areas
- Microbiology
- Genetics
- Molecular Biology
Cite this
Genetic transformation of Veillonella parvula. / Liu, Jinman; Merritt, Justin; Qi, Fengxia.
In: FEMS Microbiology Letters, Vol. 322, No. 2, 09.2011, p. 138-144.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic transformation of Veillonella parvula
AU - Liu, Jinman
AU - Merritt, Justin
AU - Qi, Fengxia
PY - 2011/9
Y1 - 2011/9
N2 - Veillonellae are one of the most prevalent and predominant microorganisms in both the supra- and subgingival plaques of the human oral cavity. Veillonellae's mutualistic relationships with the early, middle, and late colonizers of the oral cavity make them an important component of oral biofilm ecology. Unlike other ubiquitous early colonizers in the oral cavity, surprisingly little is known about Veillonella biology due to our lack of ability to genetically transform this group of bacteria. The objective of this study was to test the transformability of veillonellae. Using Veillonella parvula strain PK1910, we first obtained spontaneous mutations conferring streptomycin resistance. These mutations all carry a K43N substitution in the RpsL protein. Using the mutated rpsL gene as a selection marker, a variety of conditions were tested and optimized for electroporation. With the optimized protocol, we were able to introduce the first targeted mutation into the chromosome of V. parvula PK1910. Although more studies are needed to develop a robust genetic manipulation system in veillonellae, our results demonstrated, for the first time, that V. parvula is transformable, at least for strain PK1910.
AB - Veillonellae are one of the most prevalent and predominant microorganisms in both the supra- and subgingival plaques of the human oral cavity. Veillonellae's mutualistic relationships with the early, middle, and late colonizers of the oral cavity make them an important component of oral biofilm ecology. Unlike other ubiquitous early colonizers in the oral cavity, surprisingly little is known about Veillonella biology due to our lack of ability to genetically transform this group of bacteria. The objective of this study was to test the transformability of veillonellae. Using Veillonella parvula strain PK1910, we first obtained spontaneous mutations conferring streptomycin resistance. These mutations all carry a K43N substitution in the RpsL protein. Using the mutated rpsL gene as a selection marker, a variety of conditions were tested and optimized for electroporation. With the optimized protocol, we were able to introduce the first targeted mutation into the chromosome of V. parvula PK1910. Although more studies are needed to develop a robust genetic manipulation system in veillonellae, our results demonstrated, for the first time, that V. parvula is transformable, at least for strain PK1910.
KW - Mutagenesis
KW - Oral microbiology
KW - Veillonellae
UR - http://www.scopus.com/inward/record.url?scp=80051668914&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051668914&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2011.02344.x
DO - 10.1111/j.1574-6968.2011.02344.x
M3 - Article
C2 - 21707732
AN - SCOPUS:80051668914
VL - 322
SP - 138
EP - 144
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -