Genetic polymorphisms in head and neck cancer risk

Jeffrey E. McWilliams, Adam J. Evans, Tomasz (Tom) Beer, Peter Andersen, James Cohen, Edwin C. Everts, William David Henner

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Objective. To assess whether genetic polymorphisms implicated as risk factors for other tobacco-associated malignancies are associated with altered risk of head and neck squamous cell carcinoma. Design. Case-control study. Subjects. One hundred sixty patients with head and neck squamous cell carcinoma recruited from a university-based head and neck oncology clinic and 149 population-based controls. Methods. Genotyping of the CYP1A1 (Ile462Val), GSTM1 (null), GSTP1 (Ile105Val), GSTT1 (null), and P53 (Arg72Pro) genes was performed by polymerase chain reaction-based techniques on DNA prepared from peripheral blood. In addition, a questionnaire was used to collect demographic information from each subject. Results. Cases were significantly older (p <.0001) and had significantly greater tobacco use (p <.0001) and were more likely to be male (p <.0001) than were control subjects, thus confirming known risk factors for this disease. When cases and controls were compared by simple chi-square analysis, only the frequency of CYP1A1 (Ile462Val) polymorphism was significantly different between cases and controls (OR = .42; 95% Cl = .18-.99; p <.04). However, with a logistic regression model to control for known risk factors, we were unable to demonstrate a significant association with head and neck cancer for any of the polymorphisms tested, including CYP1A1. Conclusions. This population fails to identify a relationship between the above-mentioned polymorphisms and head and neck cancer. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)609-617
Number of pages9
JournalHead and Neck
Volume22
Issue number6
DOIs
StatePublished - 2000

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Cytochrome P-450 CYP1A1
Genetic Polymorphisms
Head and Neck Neoplasms
Logistic Models
Population Control
p53 Genes
Tobacco Use
Tobacco
Case-Control Studies
Neck
Head
Demography
Polymerase Chain Reaction
DNA
Population
Neoplasms
Carcinoma, squamous cell of head and neck

Keywords

  • CYP1A1
  • Epidemiology
  • Glutathione transferases
  • GSTM1
  • GSTP1
  • GSTT1
  • Head and neck cancer
  • P53

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Genetic polymorphisms in head and neck cancer risk. / McWilliams, Jeffrey E.; Evans, Adam J.; Beer, Tomasz (Tom); Andersen, Peter; Cohen, James; Everts, Edwin C.; Henner, William David.

In: Head and Neck, Vol. 22, No. 6, 2000, p. 609-617.

Research output: Contribution to journalArticle

McWilliams, Jeffrey E. ; Evans, Adam J. ; Beer, Tomasz (Tom) ; Andersen, Peter ; Cohen, James ; Everts, Edwin C. ; Henner, William David. / Genetic polymorphisms in head and neck cancer risk. In: Head and Neck. 2000 ; Vol. 22, No. 6. pp. 609-617.
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AB - Objective. To assess whether genetic polymorphisms implicated as risk factors for other tobacco-associated malignancies are associated with altered risk of head and neck squamous cell carcinoma. Design. Case-control study. Subjects. One hundred sixty patients with head and neck squamous cell carcinoma recruited from a university-based head and neck oncology clinic and 149 population-based controls. Methods. Genotyping of the CYP1A1 (Ile462Val), GSTM1 (null), GSTP1 (Ile105Val), GSTT1 (null), and P53 (Arg72Pro) genes was performed by polymerase chain reaction-based techniques on DNA prepared from peripheral blood. In addition, a questionnaire was used to collect demographic information from each subject. Results. Cases were significantly older (p <.0001) and had significantly greater tobacco use (p <.0001) and were more likely to be male (p <.0001) than were control subjects, thus confirming known risk factors for this disease. When cases and controls were compared by simple chi-square analysis, only the frequency of CYP1A1 (Ile462Val) polymorphism was significantly different between cases and controls (OR = .42; 95% Cl = .18-.99; p <.04). However, with a logistic regression model to control for known risk factors, we were unable to demonstrate a significant association with head and neck cancer for any of the polymorphisms tested, including CYP1A1. Conclusions. This population fails to identify a relationship between the above-mentioned polymorphisms and head and neck cancer. (C) 2000 John Wiley and Sons, Inc.

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