Genetic demonstration that the mutationally expressed nucleobase transporter of mouse S49 cells is nonconcentrative

Joanne Beck, Buddy Ullman

Research output: Contribution to journalArticle

Abstract

Somatic cell genetic analysis of purine base transporters in mouse S49 cells has demonstrated the existence of a unique high-affinity purine base transporter, which is mutationally expressed and is not found in wild-type S49 cells or any other cells of the animal kingdom (B. Aronow, et al. (1986) Mol. Cell. Biol.6, 2957). In order to determine whether this nucleobase transport system is active and concentrative, a secondary mutation in hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) was inserted into the cell line expressing this novel base transporter. The HGPRTase-deficient cells were capable of transporting hypoxanthine at increased rates but did not accumulate the base to concentrations in excess of that in the culture medium. Moreover, neither sodium azide nor ouabain had significant effects on hypoxanthine transport rates, indicating that energy metabolism and the maintenance of a sodium gradient were not required for transport function. These studies suggest that the novel mutationally expressed base transporter is independent of subsequent metabolism and does not require energy or a functioning Na+-K+-dependent ATPase activity.

Original languageEnglish (US)
Pages (from-to)254-258
Number of pages5
JournalExperimental Cell Research
Volume171
Issue number1
DOIs
StatePublished - 1987

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Hypoxanthine Phosphoribosyltransferase
Hypoxanthine
Sodium Azide
Ouabain
Energy Metabolism
Culture Media
Adenosine Triphosphatases
Sodium
Maintenance
Cell Line
Mutation
purine

ASJC Scopus subject areas

  • Cell Biology

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Genetic demonstration that the mutationally expressed nucleobase transporter of mouse S49 cells is nonconcentrative. / Beck, Joanne; Ullman, Buddy.

In: Experimental Cell Research, Vol. 171, No. 1, 1987, p. 254-258.

Research output: Contribution to journalArticle

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