Genetic correlates of morphine withdrawal in 14 inbred mouse strains

Pamela Metten; John C. Crabbe; John K. Belknap

doi:10.1016/j.drugalcdep.2008.07.006

Genetic correlates of morphine withdrawal in 14 inbred mouse strains

Pamela Metten, John C. Crabbe, John K. Belknap

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Male mice from 14 standard inbred strains were exposed to morphine in a sustained released preparation injected subcutaneously. Five hours later withdrawal was precipitated by intraperitoneal injection of naloxone. Mice were tested from 0 to 15 min after naloxone for withdrawal jumping behavior, and then from minute 15-16 for other signs, including boli count, presence of soft stool, lacrimation, "wet dog" shakes, and air chewing. They were also assessed for change in body temperature 17 min after naloxone. Strains differed markedly in the severity of withdrawal for jumping, change in body temperature, and number of fecal boli. Strains also differed in percentage of animals displaying soft stool and air chewing behavior. The other two signs were seen at too low frequency for analysis. Correlations of strain mean withdrawal severity with other responses to morphine and other abused drugs showed that high morphine withdrawal jumping and low change in body temperature were both genetically related to high morphine consumption, but not generally to other measures of morphine withdrawal or morphine sensitivity.

Original language	English (US)
Pages (from-to)	123-131
Number of pages	9
Journal	Drug and Alcohol Dependence
Volume	99
Issue number	1-3
DOIs	https://doi.org/10.1016/j.drugalcdep.2008.07.006
State	Published - Jan 1 2009
Externally published	Yes

Keywords

Dependence
Inbred mouse strains
Jumping
Morphine
Pharmacogenetics
Precipitated withdrawal

ASJC Scopus subject areas

Toxicology
Pharmacology
Psychiatry and Mental health
Pharmacology (medical)

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10.1016/j.drugalcdep.2008.07.006

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title = "Genetic correlates of morphine withdrawal in 14 inbred mouse strains",

abstract = "Male mice from 14 standard inbred strains were exposed to morphine in a sustained released preparation injected subcutaneously. Five hours later withdrawal was precipitated by intraperitoneal injection of naloxone. Mice were tested from 0 to 15 min after naloxone for withdrawal jumping behavior, and then from minute 15-16 for other signs, including boli count, presence of soft stool, lacrimation, {"}wet dog{"} shakes, and air chewing. They were also assessed for change in body temperature 17 min after naloxone. Strains differed markedly in the severity of withdrawal for jumping, change in body temperature, and number of fecal boli. Strains also differed in percentage of animals displaying soft stool and air chewing behavior. The other two signs were seen at too low frequency for analysis. Correlations of strain mean withdrawal severity with other responses to morphine and other abused drugs showed that high morphine withdrawal jumping and low change in body temperature were both genetically related to high morphine consumption, but not generally to other measures of morphine withdrawal or morphine sensitivity.",

keywords = "Dependence, Inbred mouse strains, Jumping, Morphine, Pharmacogenetics, Precipitated withdrawal",

author = "Pamela Metten and Crabbe, {John C.} and Belknap, {John K.}",

note = "Funding Information: Funding for this study was provided by NIH Grants AA10760, AA06243, AA13519, DA05228, DA10913, and two Merit Review Grants from the Department of Veterans Affairs. The NIH and the Department of Veterans Affairs had no further involvement in study design in the collection, analysis or interpretation of data, in the writing of the report, or in the decision to submit the paper for publication. ",

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N2 - Male mice from 14 standard inbred strains were exposed to morphine in a sustained released preparation injected subcutaneously. Five hours later withdrawal was precipitated by intraperitoneal injection of naloxone. Mice were tested from 0 to 15 min after naloxone for withdrawal jumping behavior, and then from minute 15-16 for other signs, including boli count, presence of soft stool, lacrimation, "wet dog" shakes, and air chewing. They were also assessed for change in body temperature 17 min after naloxone. Strains differed markedly in the severity of withdrawal for jumping, change in body temperature, and number of fecal boli. Strains also differed in percentage of animals displaying soft stool and air chewing behavior. The other two signs were seen at too low frequency for analysis. Correlations of strain mean withdrawal severity with other responses to morphine and other abused drugs showed that high morphine withdrawal jumping and low change in body temperature were both genetically related to high morphine consumption, but not generally to other measures of morphine withdrawal or morphine sensitivity.

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KW - Pharmacogenetics

KW - Precipitated withdrawal

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Genetic correlates of morphine withdrawal in 14 inbred mouse strains

Abstract

Keywords

ASJC Scopus subject areas

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