Genetic code expansion for multiprotein complex engineering

Christine Koehler, Paul F. Sauter, Mirella Wawryszyn, Gemma Estrada Girona, Kapil Gupta, Jonathan J M Landry, Markus Hsi Yang Fritz, Ksenija Radic, Jan Erik Hoffmann, Zhuo A. Chen, Juan Zou, Piau Siong Tan, Bence Galik, Sini Junttila, Peggy Stolt-Bergner, Giancarlo Pruneri, Attila Gyenesei, Carsten Schultz, Moritz Bosse Biskup, Hueseyin Besir & 7 others Vladimir Benes, Juri Rappsilber, Martin Jechlinger, Jan O. Korbel, Imre Berger, Stefan Braese, Edward A. Lemke

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodies, and (iii) for structure-function studies of novel eukaryotic complexes using single-molecule Förster resonance energy transfer as well as site-specific crosslinking strategies.

Original languageEnglish (US)
Pages (from-to)997-1000
Number of pages4
JournalNature Methods
Volume13
Issue number12
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

Fingerprint

Genetic Code
Multiprotein Complexes
Click Chemistry
Protein Engineering
Proteins
Baculoviridae
Energy Transfer
Biological Products
Production platforms
Insects
Crosslinking
Energy transfer
Labeling
Antibodies
Molecules

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Koehler, C., Sauter, P. F., Wawryszyn, M., Girona, G. E., Gupta, K., Landry, J. J. M., ... Lemke, E. A. (2016). Genetic code expansion for multiprotein complex engineering. Nature Methods, 13(12), 997-1000. https://doi.org/10.1038/nmeth.4032

Genetic code expansion for multiprotein complex engineering. / Koehler, Christine; Sauter, Paul F.; Wawryszyn, Mirella; Girona, Gemma Estrada; Gupta, Kapil; Landry, Jonathan J M; Fritz, Markus Hsi Yang; Radic, Ksenija; Hoffmann, Jan Erik; Chen, Zhuo A.; Zou, Juan; Tan, Piau Siong; Galik, Bence; Junttila, Sini; Stolt-Bergner, Peggy; Pruneri, Giancarlo; Gyenesei, Attila; Schultz, Carsten; Biskup, Moritz Bosse; Besir, Hueseyin; Benes, Vladimir; Rappsilber, Juri; Jechlinger, Martin; Korbel, Jan O.; Berger, Imre; Braese, Stefan; Lemke, Edward A.

In: Nature Methods, Vol. 13, No. 12, 01.12.2016, p. 997-1000.

Research output: Contribution to journalReview article

Koehler, C, Sauter, PF, Wawryszyn, M, Girona, GE, Gupta, K, Landry, JJM, Fritz, MHY, Radic, K, Hoffmann, JE, Chen, ZA, Zou, J, Tan, PS, Galik, B, Junttila, S, Stolt-Bergner, P, Pruneri, G, Gyenesei, A, Schultz, C, Biskup, MB, Besir, H, Benes, V, Rappsilber, J, Jechlinger, M, Korbel, JO, Berger, I, Braese, S & Lemke, EA 2016, 'Genetic code expansion for multiprotein complex engineering', Nature Methods, vol. 13, no. 12, pp. 997-1000. https://doi.org/10.1038/nmeth.4032
Koehler C, Sauter PF, Wawryszyn M, Girona GE, Gupta K, Landry JJM et al. Genetic code expansion for multiprotein complex engineering. Nature Methods. 2016 Dec 1;13(12):997-1000. https://doi.org/10.1038/nmeth.4032
Koehler, Christine ; Sauter, Paul F. ; Wawryszyn, Mirella ; Girona, Gemma Estrada ; Gupta, Kapil ; Landry, Jonathan J M ; Fritz, Markus Hsi Yang ; Radic, Ksenija ; Hoffmann, Jan Erik ; Chen, Zhuo A. ; Zou, Juan ; Tan, Piau Siong ; Galik, Bence ; Junttila, Sini ; Stolt-Bergner, Peggy ; Pruneri, Giancarlo ; Gyenesei, Attila ; Schultz, Carsten ; Biskup, Moritz Bosse ; Besir, Hueseyin ; Benes, Vladimir ; Rappsilber, Juri ; Jechlinger, Martin ; Korbel, Jan O. ; Berger, Imre ; Braese, Stefan ; Lemke, Edward A. / Genetic code expansion for multiprotein complex engineering. In: Nature Methods. 2016 ; Vol. 13, No. 12. pp. 997-1000.
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