Genetic analysis using genomic representations

Robert Lucito, Mariko Nakimura, Joseph A. West, Ying Han, Koei Chin, Kendall Jensen, Richard Mccombie, Joe W. Gray, Michael Wigler

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Analysis of the genetic changes in human tumors is often problematical because of the presence of normal stroma and the limited availability of pure tumor DNA. However, large amounts of highly reproducible 'representations' of tumor and normal genomes can be made by PCR from nanogram amounts of restriction endonuclease cleaved DNA that has been ligated to oligonucleotide adaptors. We show here that the representations are useful for many types of genetic analyses, including measuring relative gene copy numbers, loss of heterozygosity, and comparative genomic hybridization. Representations may be prepared even from sorted nuclei from fixed and archived tumor biopsies.

Original languageEnglish (US)
Pages (from-to)4487-4492
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number8
DOIs
StatePublished - Apr 14 1998

ASJC Scopus subject areas

  • General

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