Genetic analysis of the Drosophila Gsα gene

William J. Wolfgang, Ashwini Hoskote, Ian J.H. Roberts, Shannon Jackson, Michael Forte

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

One of the best understood signal transduction pathways activated by receptors containing seven transmembrane domains involves activation of heterotrimeric G-protein complexes containing Gsα, the subsequent stimulation of adenylyl cyclase, production of cAMP, activation of protein kinase A (PKA), and the phosphorylation of substrates that control a wide variety of cellular responses. Here, we report the identification of "loss-of-function" mutations in the Drosophila Gsα gene (dgs). Seven mutants have been identified that are either complemented by transgenes representing the wild-type dgs gene or contain nucleotide sequence changes resulting in the production of altered Gsα protein. Examination of mutant alleles representing loss-of-Gsα function indicates that the phenotypes generated do not mimic those created by mutational elimination of PKA. These results are consistent with the conclusion reached in previous studies that activation of PKA, at least in these developmental contexts, does not depend on receptor-mediated increases in intracellular cAMP, in contrast to the predictions of models developed primarily on the basis of studies in cultured cells.

Original languageEnglish (US)
Pages (from-to)1189-1201
Number of pages13
JournalGenetics
Volume158
Issue number3
StatePublished - Aug 1 2001

ASJC Scopus subject areas

  • Genetics

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