TY - JOUR
T1 - Genetic analysis of the cell-to-cell movement of beet yellows closterovirus
AU - Alzhanova, Dina V.
AU - Hagiwara, Yuka
AU - Peremyslov, Valery V.
AU - Dolja, Valerian V.
N1 - Funding Information:
We thank William Dawson for useful discussions and for sharing unpublished data, Eugene Koonin for his help with computer analysis of amino acid sequences, and Theo Dreher for critical reading of the manuscript. We are grateful to Jonathan Reed and George Gvakharia for the excellent technical assistance. This work was supported by grants from the National Institutes of Health (R1GM53190B) and from the U.S. Department of Agriculture (NRICGP 97-35303-4515) to V.V.D.
PY - 2000/3/1
Y1 - 2000/3/1
N2 - A beet yellows closterovirus (BYV) variant expressing green fluorescent protein and leaves of BYV local lesion host Claytonia perfoliata were used to reveal genetic requirements for BYV cell-to-cell movement in leaf epidermis and mesophyll A series of mutations targeting genes that a re not involved in amplification of the viral positive-strand RNA was analyzed. The products of genes coding for a 6-kDa hydrophobic protein (p6) and a 64-kDa protein (p64), as well as for minor and major capsid proteins, were found to be essential for intercellular translocation of BYV. In a previous work, we have demonstrated that the BYV HSP70-homolog (HSP70h) also plays a critical role in viral movement (V. V. Peremyslov, Y. Hagiwara, and V. V. Dolja, 1999, Proc. Natl. Acad. Sci. USA, 96, 14771-14776). Altogether, a unique protein quintet including three dedicated movement proteins (p6, p64, and HSP70h) and two structural proteins is required to potentiate the cell-to- cell movement of a closterovirus. The corresponding BYV genes are clustered in a block that is conserved among diverse representatives of the family Closteroviridae. (C) 2000 Academic Press.
AB - A beet yellows closterovirus (BYV) variant expressing green fluorescent protein and leaves of BYV local lesion host Claytonia perfoliata were used to reveal genetic requirements for BYV cell-to-cell movement in leaf epidermis and mesophyll A series of mutations targeting genes that a re not involved in amplification of the viral positive-strand RNA was analyzed. The products of genes coding for a 6-kDa hydrophobic protein (p6) and a 64-kDa protein (p64), as well as for minor and major capsid proteins, were found to be essential for intercellular translocation of BYV. In a previous work, we have demonstrated that the BYV HSP70-homolog (HSP70h) also plays a critical role in viral movement (V. V. Peremyslov, Y. Hagiwara, and V. V. Dolja, 1999, Proc. Natl. Acad. Sci. USA, 96, 14771-14776). Altogether, a unique protein quintet including three dedicated movement proteins (p6, p64, and HSP70h) and two structural proteins is required to potentiate the cell-to- cell movement of a closterovirus. The corresponding BYV genes are clustered in a block that is conserved among diverse representatives of the family Closteroviridae. (C) 2000 Academic Press.
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U2 - 10.1006/viro.1999.0155
DO - 10.1006/viro.1999.0155
M3 - Article
C2 - 10683341
AN - SCOPUS:0034007010
SN - 0042-6822
VL - 268
SP - 192
EP - 200
JO - Virology
JF - Virology
IS - 1
ER -