Genetic analysis of the cell-to-cell movement of beet yellows closterovirus

Dina Alzhanova, Yuka Hagiwara, Valery V. Peremyslov, Valerian V. Dolja

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

A beet yellows closterovirus (BYV) variant expressing green fluorescent protein and leaves of BYV local lesion host Claytonia perfoliata were used to reveal genetic requirements for BYV cell-to-cell movement in leaf epidermis and mesophyll A series of mutations targeting genes that a re not involved in amplification of the viral positive-strand RNA was analyzed. The products of genes coding for a 6-kDa hydrophobic protein (p6) and a 64-kDa protein (p64), as well as for minor and major capsid proteins, were found to be essential for intercellular translocation of BYV. In a previous work, we have demonstrated that the BYV HSP70-homolog (HSP70h) also plays a critical role in viral movement (V. V. Peremyslov, Y. Hagiwara, and V. V. Dolja, 1999, Proc. Natl. Acad. Sci. USA, 96, 14771-14776). Altogether, a unique protein quintet including three dedicated movement proteins (p6, p64, and HSP70h) and two structural proteins is required to potentiate the cell-to- cell movement of a closterovirus. The corresponding BYV genes are clustered in a block that is conserved among diverse representatives of the family Closteroviridae. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)192-200
Number of pages9
JournalVirology
Volume268
Issue number1
DOIs
StatePublished - Mar 1 2000

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Closterovirus
Beta vulgaris
Cell Movement
Portulacaceae
Closteroviridae
Protein Structural Homology
Proteins
Gene Targeting
Capsid Proteins
Green Fluorescent Proteins
Epidermis
Genes
RNA
Mutation

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Alzhanova, D., Hagiwara, Y., Peremyslov, V. V., & Dolja, V. V. (2000). Genetic analysis of the cell-to-cell movement of beet yellows closterovirus. Virology, 268(1), 192-200. https://doi.org/10.1006/viro.1999.0155

Genetic analysis of the cell-to-cell movement of beet yellows closterovirus. / Alzhanova, Dina; Hagiwara, Yuka; Peremyslov, Valery V.; Dolja, Valerian V.

In: Virology, Vol. 268, No. 1, 01.03.2000, p. 192-200.

Research output: Contribution to journalArticle

Alzhanova, D, Hagiwara, Y, Peremyslov, VV & Dolja, VV 2000, 'Genetic analysis of the cell-to-cell movement of beet yellows closterovirus', Virology, vol. 268, no. 1, pp. 192-200. https://doi.org/10.1006/viro.1999.0155
Alzhanova D, Hagiwara Y, Peremyslov VV, Dolja VV. Genetic analysis of the cell-to-cell movement of beet yellows closterovirus. Virology. 2000 Mar 1;268(1):192-200. https://doi.org/10.1006/viro.1999.0155
Alzhanova, Dina ; Hagiwara, Yuka ; Peremyslov, Valery V. ; Dolja, Valerian V. / Genetic analysis of the cell-to-cell movement of beet yellows closterovirus. In: Virology. 2000 ; Vol. 268, No. 1. pp. 192-200.
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