Genetic analysis of the cell-to-cell movement of beet yellows closterovirus

Dina V. Alzhanova, Yuka Hagiwara, Valery V. Peremyslov, Valerian V. Dolja

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

A beet yellows closterovirus (BYV) variant expressing green fluorescent protein and leaves of BYV local lesion host Claytonia perfoliata were used to reveal genetic requirements for BYV cell-to-cell movement in leaf epidermis and mesophyll A series of mutations targeting genes that a re not involved in amplification of the viral positive-strand RNA was analyzed. The products of genes coding for a 6-kDa hydrophobic protein (p6) and a 64-kDa protein (p64), as well as for minor and major capsid proteins, were found to be essential for intercellular translocation of BYV. In a previous work, we have demonstrated that the BYV HSP70-homolog (HSP70h) also plays a critical role in viral movement (V. V. Peremyslov, Y. Hagiwara, and V. V. Dolja, 1999, Proc. Natl. Acad. Sci. USA, 96, 14771-14776). Altogether, a unique protein quintet including three dedicated movement proteins (p6, p64, and HSP70h) and two structural proteins is required to potentiate the cell-to- cell movement of a closterovirus. The corresponding BYV genes are clustered in a block that is conserved among diverse representatives of the family Closteroviridae. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)192-200
Number of pages9
JournalVirology
Volume268
Issue number1
DOIs
StatePublished - Mar 1 2000

ASJC Scopus subject areas

  • Virology

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    Alzhanova, D. V., Hagiwara, Y., Peremyslov, V. V., & Dolja, V. V. (2000). Genetic analysis of the cell-to-cell movement of beet yellows closterovirus. Virology, 268(1), 192-200. https://doi.org/10.1006/viro.1999.0155