TY - JOUR
T1 - Genetic analysis of HIV-1 Circulating Recombinant Form 02_AG, B and C subtype-specific envelope sequences from Northern India and their predicted co-receptor usage
AU - Neogi, Ujjwal
AU - Sood, Vikas
AU - Chowdhury, Arpita
AU - Das, Shukla
AU - Ramachandran, Vishnampettai G.
AU - Sreedhar, Vijesh K.
AU - Wanchu, Ajay
AU - Ghosh, Nilanjana
AU - Banerjea, Akhil C.
N1 - Funding Information:
This study was supported by grants from Department of Biotechnology (DBT), Indian Council of Medical Research and National Institutes of Health, USA, to ACB, AW and to the National Institute of Immunology, New Delhi. This work was partially supported by the National Bio-Science Award of DBT to ACB.
PY - 2009/12/3
Y1 - 2009/12/3
N2 - HIV-1 epidemic in India is largely driven by subtype C but other subtypes or recombinants have also been reported from several states of India. This is mainly due to the co-circulation of other genetic subtypes that potentially can recombine to generate recombinant/mosaic genomes. In this study, we report detail genetic characterization of HIV-1 envelope sequences from North India (Delhi and neighboring regions). Six of 13 were related to subtype C, one B and the rest six showed relatedness with CRF02_AG strain. The subtype C possessed the highly conserved GPGQ motif but subtype B possessed the GPGR motif in the V3 loop as observed earlier. While most of the sequences suggested CCR5 co-receptor usage, one subtype C sample clearly indicated CXCR4 usage. A successful mother to child transmission was established in two pairs. Thus, co-circulation of multiple subtypes (B and C) and the recombinant CRF02_AG strains in North India suggests a rapidly evolving scenario of HIV-1 epidemic in this region with impact on vaccine formulation. Since this is the first report of CRF02_AG envelope from India, it will be important to monitor the spread of this strain and its impact on HIV-1 transmission in India.
AB - HIV-1 epidemic in India is largely driven by subtype C but other subtypes or recombinants have also been reported from several states of India. This is mainly due to the co-circulation of other genetic subtypes that potentially can recombine to generate recombinant/mosaic genomes. In this study, we report detail genetic characterization of HIV-1 envelope sequences from North India (Delhi and neighboring regions). Six of 13 were related to subtype C, one B and the rest six showed relatedness with CRF02_AG strain. The subtype C possessed the highly conserved GPGQ motif but subtype B possessed the GPGR motif in the V3 loop as observed earlier. While most of the sequences suggested CCR5 co-receptor usage, one subtype C sample clearly indicated CXCR4 usage. A successful mother to child transmission was established in two pairs. Thus, co-circulation of multiple subtypes (B and C) and the recombinant CRF02_AG strains in North India suggests a rapidly evolving scenario of HIV-1 epidemic in this region with impact on vaccine formulation. Since this is the first report of CRF02_AG envelope from India, it will be important to monitor the spread of this strain and its impact on HIV-1 transmission in India.
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U2 - 10.1186/1742-6405-6-28
DO - 10.1186/1742-6405-6-28
M3 - Article
C2 - 19954551
AN - SCOPUS:77955513047
SN - 1742-6405
VL - 6
JO - AIDS Research and Therapy
JF - AIDS Research and Therapy
M1 - 28
ER -