Genes involved in the repression of mutacin I production in Streptococcus mutans

Trang Nguyen, Zhijun Zhang, I. Hsiu Huang, Chenggang Wu, Justin Merritt, Wenyuan Shi, Fengxia Qi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Streptococcus mutans is considered a primary pathogen for human dental caries. Its ability to produce a variety of peptide antibiotics called mutacins may play an important role in its invasion and establishment in the dental biofilm. S. mutans strain UA140 produces two types of mutacins, the lantibiotic mutacin I and the non-lantibiotic mutacin IV. In a previous study, we constructed a random insertional-mutation library to screen for genes involved in regulating mutacin I production, and found 25 genes/operons that have a positive effect on mutacin I production. In this study, we continued our previous work to identify genes that are negatively involved in mutacin I production. By using a high-phosphate brain heart infusion agar medium that inhibited mutacin I production of the wild-type, we isolated 77 clones that consistently produced mutacin I under repressive conditions. From the 34 clones for which we were able to obtain a sequence, 17 unique genes were identified. These genes encompass a variety of functional groups, including central metabolism, surface binding and sugar transport, and unknown functions. Some of the 17 mutations were further characterized and shown to increase mutacin gene expression during growth when the gene is usually not expressed in the wild-type. These results further demonstrate an intimate and intricate connection between mutacin production and the overall cellular homeostasis.

Original languageEnglish (US)
Pages (from-to)551-556
Number of pages6
JournalMicrobiology
Volume155
Issue number2
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint

Streptococcus mutans
Genes
Clone Cells
Bacteriocins
Mutation
Dental Caries
Operon
Biofilms
Libraries
Agar
mutacin I
Tooth
Homeostasis
Phosphates
Anti-Bacterial Agents
Gene Expression
Peptides
Brain
Growth

ASJC Scopus subject areas

  • Microbiology

Cite this

Genes involved in the repression of mutacin I production in Streptococcus mutans. / Nguyen, Trang; Zhang, Zhijun; Huang, I. Hsiu; Wu, Chenggang; Merritt, Justin; Shi, Wenyuan; Qi, Fengxia.

In: Microbiology, Vol. 155, No. 2, 01.01.2009, p. 551-556.

Research output: Contribution to journalArticle

Nguyen, T, Zhang, Z, Huang, IH, Wu, C, Merritt, J, Shi, W & Qi, F 2009, 'Genes involved in the repression of mutacin I production in Streptococcus mutans', Microbiology, vol. 155, no. 2, pp. 551-556. https://doi.org/10.1099/mic.0.021303-0
Nguyen, Trang ; Zhang, Zhijun ; Huang, I. Hsiu ; Wu, Chenggang ; Merritt, Justin ; Shi, Wenyuan ; Qi, Fengxia. / Genes involved in the repression of mutacin I production in Streptococcus mutans. In: Microbiology. 2009 ; Vol. 155, No. 2. pp. 551-556.
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