Genes, behavior and next-generation RNA sequencing

Robert Hitzemann, D. Bottomly, P. Darakjian, N. Walter, Ovidiu Iancu, Robert Searles, Beth Wilmot, Shannon McWeeney

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Advances in next-generation sequencing suggest that RNA-Seq is poised to supplant microarray-based approaches for transcriptome analysis. This article briefly reviews the use of microarrays in the brain-behavior context and then illustrates why RNA-Seq is a superior strategy. Compared with microarrays, RNA-Seq has a greater dynamic range, detects both coding and noncoding RNAs, is superior for gene network construction, detects alternative spliced transcripts, detects allele specific expression and can be used to extract genotype information, e.g. nonsynonymous coding single nucleotide polymorphisms. Examples of where RNA-Seq has been used to assess brain gene expression are provided. Despite the advantages of RNA-Seq, some disadvantages remain. These include the high cost of RNA-Seq and the computational complexities associated with data analysis. RNA-Seq embraces the complexity of the transcriptome and provides a mechanism to understand the underlying regulatory code; the potential to inform the brain-behavior relationship is substantial.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalGenes, Brain and Behavior
Volume12
Issue number1
DOIs
StatePublished - Feb 2013

Fingerprint

RNA Sequence Analysis
RNA
Genes
Brain
Untranslated RNA
Gene Regulatory Networks
Gene Expression Profiling
Transcriptome
Single Nucleotide Polymorphism
Alleles
Genotype
Gene Expression
Costs and Cost Analysis

Keywords

  • Behavior
  • Brain
  • Genetics
  • Microarrays
  • RNA-Seq

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Genetics
  • Neurology

Cite this

Genes, behavior and next-generation RNA sequencing. / Hitzemann, Robert; Bottomly, D.; Darakjian, P.; Walter, N.; Iancu, Ovidiu; Searles, Robert; Wilmot, Beth; McWeeney, Shannon.

In: Genes, Brain and Behavior, Vol. 12, No. 1, 02.2013, p. 1-12.

Research output: Contribution to journalArticle

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