Abstract
AE3 gene is a member of the AE anion exchanger gene family that is expressed primarily in brain and heart. The principal product of the AE3 gene in rodent brain, FL-AE3p, when expressed in heterologous cell lines, gives rise to chloride-dependent changes in intracellular pH consistent with its operation as an anion exchanger. We have identified two novel isoforms of mouse AE3 that are generated by tissue-specific alternate RNA processing. One of these isoforms encodes a polypeptide, 14-AE3p, that corresponds to a portion of the NH2-terminal cytoplasmic domain of AE3. 14-AE3p lacks the entire transmembrane domain that - in FL-AE3p forms the anion exchange channel. Immunoblots with antibodies to the NH2- and COOH- termini confirm that FL-AE3 and 14-AE3 are expressed in rat brain as 160 kDa and 74 kDa polypeptides, respectively. Unlike FL-AE3p, however, 14-AE3p is insoluble in non-ionic detergent, suggesting a possible association with the cytoskeleton.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1275-1282 |
| Number of pages | 8 |
| Journal | Journal of Cell Science |
| Volume | 106 |
| Issue number | 4 |
| State | Published - 1993 |
| Externally published | Yes |
Keywords
- AE3
- Alternate splicing
- Anion exchanger
- Cytoskeleton
ASJC Scopus subject areas
- Cell Biology