Generation of truncated brain AE3 isoforms by alternate mRNA processing

C. W. Morgans, R. R. Kopito

    Research output: Contribution to journalArticle

    14 Scopus citations

    Abstract

    AE3 gene is a member of the AE anion exchanger gene family that is expressed primarily in brain and heart. The principal product of the AE3 gene in rodent brain, FL-AE3p, when expressed in heterologous cell lines, gives rise to chloride-dependent changes in intracellular pH consistent with its operation as an anion exchanger. We have identified two novel isoforms of mouse AE3 that are generated by tissue-specific alternate RNA processing. One of these isoforms encodes a polypeptide, 14-AE3p, that corresponds to a portion of the NH2-terminal cytoplasmic domain of AE3. 14-AE3p lacks the entire transmembrane domain that - in FL-AE3p forms the anion exchange channel. Immunoblots with antibodies to the NH2- and COOH- termini confirm that FL-AE3 and 14-AE3 are expressed in rat brain as 160 kDa and 74 kDa polypeptides, respectively. Unlike FL-AE3p, however, 14-AE3p is insoluble in non-ionic detergent, suggesting a possible association with the cytoskeleton.

    Original languageEnglish (US)
    Pages (from-to)1275-1282
    Number of pages8
    JournalJournal of Cell Science
    Volume106
    Issue number4
    StatePublished - Dec 1 1993

    Keywords

    • AE3
    • Alternate splicing
    • Anion exchanger
    • Cytoskeleton

    ASJC Scopus subject areas

    • Cell Biology

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