Generation of truncated brain AE3 isoforms by alternate mRNA processing

C. W. Morgans, R. R. Kopito

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

AE3 gene is a member of the AE anion exchanger gene family that is expressed primarily in brain and heart. The principal product of the AE3 gene in rodent brain, FL-AE3p, when expressed in heterologous cell lines, gives rise to chloride-dependent changes in intracellular pH consistent with its operation as an anion exchanger. We have identified two novel isoforms of mouse AE3 that are generated by tissue-specific alternate RNA processing. One of these isoforms encodes a polypeptide, 14-AE3p, that corresponds to a portion of the NH2-terminal cytoplasmic domain of AE3. 14-AE3p lacks the entire transmembrane domain that - in FL-AE3p forms the anion exchange channel. Immunoblots with antibodies to the NH2- and COOH- termini confirm that FL-AE3 and 14-AE3 are expressed in rat brain as 160 kDa and 74 kDa polypeptides, respectively. Unlike FL-AE3p, however, 14-AE3p is insoluble in non-ionic detergent, suggesting a possible association with the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)1275-1282
Number of pages8
JournalJournal of Cell Science
Volume106
Issue number4
StatePublished - Dec 1 1993
Externally publishedYes

Keywords

  • AE3
  • Alternate splicing
  • Anion exchanger
  • Cytoskeleton

ASJC Scopus subject areas

  • Cell Biology

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