Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells

Meghan Cansler, Megan Null, Erin Meermeier, Gwendolyn Swarbrick, David Lewinsohn, Melanie Harriff

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Tetramers are a powerful tool for identification of T cell subsets that are restricted by specific antigen presenting molecules and their cognate antigens. The generation of T cell clones from specific T cell subsets allows for further investigation of the phenotype and function of these cells. Here, we describe a method for sorting and cloning of MR1-restricted T cells using the MR1/5-OP-RU tetramer. This protocol can be easily modified to enrich for expansion of specific or unique subsets of MR1-restricted T cell clones from any tissue to further characterize the phenotype and function of those cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • MAIT cells
  • MR1-restricted T cells
  • T cell cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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