@inbook{48248f0bfb194057b4c2dd1aca80d4d2,
title = "Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells",
abstract = "Tetramers are a powerful tool for identification of T cell subsets that are restricted by specific antigen presenting molecules and their cognate antigens. The generation of T cell clones from specific T cell subsets allows for further investigation of the phenotype and function of these cells. Here, we describe a method for sorting and cloning of MR1-restricted T cells using the MR1/5-OP-RU tetramer. This protocol can be easily modified to enrich for expansion of specific or unique subsets of MR1-restricted T cell clones from any tissue to further characterize the phenotype and function of those cells.",
keywords = "MAIT cells, MR1-restricted T cells, T cell cloning",
author = "Meghan Cansler and Megan Null and Erin Meermeier and Gwendolyn Swarbrick and David Lewinsohn and Melanie Harriff",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0207-2_15",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "219--235",
booktitle = "Methods in Molecular Biology",
}