Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells

Meghan Cansler, Megan Null, Erin Meermeier, Gwendolyn Swarbrick, David Lewinsohn, Melanie Harriff

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Tetramers are a powerful tool for identification of T cell subsets that are restricted by specific antigen presenting molecules and their cognate antigens. The generation of T cell clones from specific T cell subsets allows for further investigation of the phenotype and function of these cells. Here, we describe a method for sorting and cloning of MR1-restricted T cells using the MR1/5-OP-RU tetramer. This protocol can be easily modified to enrich for expansion of specific or unique subsets of MR1-restricted T cell clones from any tissue to further characterize the phenotype and function of those cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages219-235
Number of pages17
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2098
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Organism Cloning
Clone Cells
T-Lymphocyte Subsets
T-Lymphocytes
Phenotype
Antigens
caN protocol

Keywords

  • MAIT cells
  • MR1-restricted T cells
  • T cell cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Cansler, M., Null, M., Meermeier, E., Swarbrick, G., Lewinsohn, D., & Harriff, M. (2020). Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells. In Methods in Molecular Biology (pp. 219-235). (Methods in Molecular Biology; Vol. 2098). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0207-2_15

Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells. / Cansler, Meghan; Null, Megan; Meermeier, Erin; Swarbrick, Gwendolyn; Lewinsohn, David; Harriff, Melanie.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 219-235 (Methods in Molecular Biology; Vol. 2098).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cansler, M, Null, M, Meermeier, E, Swarbrick, G, Lewinsohn, D & Harriff, M 2020, Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2098, Humana Press Inc., pp. 219-235. https://doi.org/10.1007/978-1-0716-0207-2_15
Cansler M, Null M, Meermeier E, Swarbrick G, Lewinsohn D, Harriff M. Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 219-235. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-0716-0207-2_15
Cansler, Meghan ; Null, Megan ; Meermeier, Erin ; Swarbrick, Gwendolyn ; Lewinsohn, David ; Harriff, Melanie. / Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 219-235 (Methods in Molecular Biology).
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