Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells

Meghan Cansler, Megan Null, Erin Meermeier, Gwendolyn Swarbrick, David Lewinsohn, Melanie Harriff

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Tetramers are a powerful tool for identification of T cell subsets that are restricted by specific antigen presenting molecules and their cognate antigens. The generation of T cell clones from specific T cell subsets allows for further investigation of the phenotype and function of these cells. Here, we describe a method for sorting and cloning of MR1-restricted T cells using the MR1/5-OP-RU tetramer. This protocol can be easily modified to enrich for expansion of specific or unique subsets of MR1-restricted T cell clones from any tissue to further characterize the phenotype and function of those cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages219-235
Number of pages17
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2098
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • MAIT cells
  • MR1-restricted T cells
  • T cell cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer<sup>+</sup> Cells'. Together they form a unique fingerprint.

  • Cite this

    Cansler, M., Null, M., Meermeier, E., Swarbrick, G., Lewinsohn, D., & Harriff, M. (2020). Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer+ Cells. In Methods in Molecular Biology (pp. 219-235). (Methods in Molecular Biology; Vol. 2098). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0207-2_15