Generation of mice with a conditional allele for Ift172

Paul W. Howard, Tiffani L. Howard, Richard A. Maurer

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Ift172 encodes a gene product that is part of a complex that mediates intraflagellar transport (IFT), a process necessary for the genesis and maintenance of cilia. Genetic studies in mice have offered evidence that Ift172 also plays a role in hedgehog signaling. Disruption of Ift172 in mice is associated with lethality at about embryonic day 11, limiting studies to understand the role for Ift172 in later development and the adult. To further our understanding of the later roles of Ift172, we have generated mice with a conditional allele for Ift172. We have confirmed the phenotype of the disrupted allele by using CRE expression directed by the prx1 enhancer to disrupt the conditional Ift172 allele in the developing limb.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalTransgenic Research
Volume19
Issue number1
DOIs
StatePublished - Jan 1 2010

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Keywords

  • CRE recombinase
  • Hedgehog
  • Intraflagellar transport
  • Limb development

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

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