Generation of mice with a conditional allele for Ift172

Paul W. Howard, Tiffani L. Howard, Richard Maurer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ift172 encodes a gene product that is part of a complex that mediates intraflagellar transport (IFT), a process necessary for the genesis and maintenance of cilia. Genetic studies in mice have offered evidence that Ift172 also plays a role in hedgehog signaling. Disruption of Ift172 in mice is associated with lethality at about embryonic day 11, limiting studies to understand the role for Ift172 in later development and the adult. To further our understanding of the later roles of Ift172, we have generated mice with a conditional allele for Ift172. We have confirmed the phenotype of the disrupted allele by using CRE expression directed by the prx1 enhancer to disrupt the conditional Ift172 allele in the developing limb.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalTransgenic Research
Volume19
Issue number1
DOIs
StatePublished - Jan 2010

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Alleles
alleles
mice
Hedgehogs
Erinaceidae
Cilia
cilia
Extremities
Maintenance
Phenotype
phenotype
Genes
genes

Keywords

  • CRE recombinase
  • Hedgehog
  • Intraflagellar transport
  • Limb development

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Agronomy and Crop Science
  • Animal Science and Zoology

Cite this

Generation of mice with a conditional allele for Ift172. / Howard, Paul W.; Howard, Tiffani L.; Maurer, Richard.

In: Transgenic Research, Vol. 19, No. 1, 01.2010, p. 121-126.

Research output: Contribution to journalArticle

Howard, Paul W. ; Howard, Tiffani L. ; Maurer, Richard. / Generation of mice with a conditional allele for Ift172. In: Transgenic Research. 2010 ; Vol. 19, No. 1. pp. 121-126.
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