Generation of cytotoxic lymphocytes to syngeneic tumors by using co-stimulator (Interleukin 2): In vivo activity

G. B. Mills, G. Carlson, V. Paetkau

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Spleen cells from DBA/2J mice bearing the syngeneic tumor mastocytoma P815, incubated with co-stimulator (Interleukin 2) and P815 in vitro, were effective in killing P815 cells in vivo. Within 24 hr of injection, 1 x 107 cytotoxic lymphocytes (CL) killed most of 1 x 106 P815 cells. The host response to the tumor 7 to 9 days after the initial tumor injection was also greatly enhanced in mice that had received CL. This effect was potentiated in sublethally irradiated mice. CL were effective in mice with large tumors, overcoming suppressive factors that might be present. Under certain conditions, a significant fraction of CL-treated mice survived the P815 tumor indefinitely. These included i.p. CL given 2 days after a large dose (1 x 106) of i.p. P815. In addition, some mice given i.v. and intra-tumor CL survived small doses (1 x 104) of subcutaneous P815 cells. Long-term surviving mice remained resistant to challenge with 1 x 106 tumor cells (which is 104 times the normally lethal dose) for at least 1 yr. No detrimental effects were noted even after injection of 5 x 107 CL per mouse.

Original languageEnglish (US)
Pages (from-to)1904-1909
Number of pages6
JournalJournal of Immunology
Volume125
Issue number5
StatePublished - Jan 1 1980
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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