Generation of cyclic guanosine monophosphate in brain slices incubated with atrial or C-type natriuretic peptides: comparison of the amplitudes and cellular distribution of the responses

Jason Gonçalves, Kevin Grove, Christian F. Deschepper

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Natriuretic peptides have been demonstrated to induce a variety of effects when administered into the brain. Most studies to date have tested the effects of 'atrial' natriuretic peptide (ANP), but C-type natriuretic peptide (CNP) has recently been suggested to be the predominant form of natriuretic peptides within the brain. We therefore have compared the amplitudes of the cyclic guanosine monophosphate (cGMP) responses induced by either ANP or CNP in slices from different rat brain regions. Whereas both peptides induced the generation of cGMP, CNP-evoked responses were never greater than those obtained with ANP, regardless of the brain region used or the age of the animal. In diencephalon, ANP even induced a significantly higher cGMP response than CNP. To test which cells were targets to the actions of the peptides, brain slices were incubated with fluorocitrate (a drug that selectively blocks the metabolism of glial cells). Fluorocitrate totally blocked the ANP-evoked cGMP responses in brain slices. In contrast, fluorocitrate reduced only partially the responses evoked by sodium nitroprusside (a drug that stimulates soluble guanylate cyclase, which is contained predominantly in neurons). Likewise, the cGMP response induced by CNP was only partially affected by fluorocitrate. These results indicate that: (1) CNP is not more potent than ANP in terms of its ability to generate cGMP in rat brains; (2) brain cells generating cGMP upon exposure to ANP are predominantly glial; and (3) CNP-responsive cells are partly glial, but belong at least in part to a different compartment than ANP-responsive cells. All together, these data suggest that there is a functional separation between the effects of ANP and CNP in rat brains.

Original languageEnglish (US)
Pages (from-to)55-63
Number of pages9
JournalRegulatory Peptides
Volume57
Issue number1
DOIs
StatePublished - May 4 1995
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Cyclic GMP
Atrial Natriuretic Factor
Brain
Neuroglia
Rats
Natriuretic Peptides
Diencephalon
Peptides
Guanylate Cyclase
Brain Natriuretic Peptide
Nitroprusside
Metabolism
Pharmaceutical Preparations
Neurons
Animals

Keywords

  • Atrial natriuretic peptide (ANP)
  • Brain
  • C-type natriuretic peptide (CNP)
  • Cyclic guanosine monophosphate (cGMP)
  • Fluorocitrate
  • Glial cells

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience
  • Neuroscience(all)

Cite this

Generation of cyclic guanosine monophosphate in brain slices incubated with atrial or C-type natriuretic peptides : comparison of the amplitudes and cellular distribution of the responses. / Gonçalves, Jason; Grove, Kevin; Deschepper, Christian F.

In: Regulatory Peptides, Vol. 57, No. 1, 04.05.1995, p. 55-63.

Research output: Contribution to journalArticle

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