Generation of chimeric rhesus monkeys

Masahito Tachibana, Michelle Sparman, Cathy Ramsey, Hong Ma, Hyo Sang Lee, Maria Cecilia T. Penedo, Shoukhrat Mitalipov

    Research output: Contribution to journalArticlepeer-review

    124 Scopus citations

    Abstract

    Totipotent cells in early embryos are progenitors of all stem cells and are capable of developing into a whole organism, including extraembryonic tissues such as placenta. Pluripotent cells in the inner cell mass (ICM) are the descendants of totipotent cells and can differentiate into any cell type of a body except extraembryonic tissues. The ability to contribute to chimeric animals upon reintroduction into host embryos is the key feature of murine totipotent and pluripotent cells. Here, we demonstrate that rhesus monkey embryonic stem cells (ESCs) and isolated ICMs fail to incorporate into host embryos and develop into chimeras. However, chimeric offspring were produced following aggregation of totipotent cells of the four-cell embryos. These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible.

    Original languageEnglish (US)
    Pages (from-to)285-295
    Number of pages11
    JournalCell
    Volume148
    Issue number1-2
    DOIs
    StatePublished - Jan 20 2012

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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