TY - JOUR
T1 - Generation of chimeric rhesus monkeys
AU - Tachibana, Masahito
AU - Sparman, Michelle
AU - Ramsey, Cathy
AU - Ma, Hong
AU - Lee, Hyo Sang
AU - Penedo, Maria Cecilia T.
AU - Mitalipov, Shoukhrat
N1 - Funding Information:
The authors would like to acknowledge the Assisted Reproductive Technology & Embryonic Stem Cell Core, Division of Animal Resources, Surgery Team, Endocrine Technology Core, and Molecular & Cellular Biology Core at the Oregon National Primate Research Center for providing expertise and services that contributed to this project. We are grateful to Dr. Warren Sanger from Human Cytogenetic Laboratory, Nebraska Medical Center for proving karyotyping services. We are obliged to Hathaitip Sritanaudomchai, Keith Masterson, Lisa Clepper, Joy Woodward, Maidina Touhetahuntila, and Erin Wolff for their technical support, Joel Ito for help with illustrative materials, and Dr. Mary Herbert for providing a GFP-RNA vector. We are indebted to Drs. Richard Stouffer and Don Wolf for consulting, helpful discussions, and critical reading of the manuscript. This study was supported by startup funds from the Oregon National Primate Research Center and grants from the National Institutes of Health, HD057121, HD059946, HD063276, HD047675, HD018185, and RR000163.
PY - 2012/1/20
Y1 - 2012/1/20
N2 - Totipotent cells in early embryos are progenitors of all stem cells and are capable of developing into a whole organism, including extraembryonic tissues such as placenta. Pluripotent cells in the inner cell mass (ICM) are the descendants of totipotent cells and can differentiate into any cell type of a body except extraembryonic tissues. The ability to contribute to chimeric animals upon reintroduction into host embryos is the key feature of murine totipotent and pluripotent cells. Here, we demonstrate that rhesus monkey embryonic stem cells (ESCs) and isolated ICMs fail to incorporate into host embryos and develop into chimeras. However, chimeric offspring were produced following aggregation of totipotent cells of the four-cell embryos. These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible.
AB - Totipotent cells in early embryos are progenitors of all stem cells and are capable of developing into a whole organism, including extraembryonic tissues such as placenta. Pluripotent cells in the inner cell mass (ICM) are the descendants of totipotent cells and can differentiate into any cell type of a body except extraembryonic tissues. The ability to contribute to chimeric animals upon reintroduction into host embryos is the key feature of murine totipotent and pluripotent cells. Here, we demonstrate that rhesus monkey embryonic stem cells (ESCs) and isolated ICMs fail to incorporate into host embryos and develop into chimeras. However, chimeric offspring were produced following aggregation of totipotent cells of the four-cell embryos. These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible.
UR - http://www.scopus.com/inward/record.url?scp=84856111921&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856111921&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2011.12.007
DO - 10.1016/j.cell.2011.12.007
M3 - Article
C2 - 22225614
AN - SCOPUS:84856111921
SN - 0092-8674
VL - 148
SP - 285
EP - 295
JO - Cell
JF - Cell
IS - 1-2
ER -