Generation of chimeric rhesus monkeys

Masahito Tachibana, Michelle Sparman, Cathy Ramsey, Hong Ma, Hyo Sang Lee, Maria Cecilia T Penedo, Shoukhrat Mitalipov

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Totipotent cells in early embryos are progenitors of all stem cells and are capable of developing into a whole organism, including extraembryonic tissues such as placenta. Pluripotent cells in the inner cell mass (ICM) are the descendants of totipotent cells and can differentiate into any cell type of a body except extraembryonic tissues. The ability to contribute to chimeric animals upon reintroduction into host embryos is the key feature of murine totipotent and pluripotent cells. Here, we demonstrate that rhesus monkey embryonic stem cells (ESCs) and isolated ICMs fail to incorporate into host embryos and develop into chimeras. However, chimeric offspring were produced following aggregation of totipotent cells of the four-cell embryos. These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible.

Original languageEnglish (US)
Pages (from-to)285-295
Number of pages11
JournalCell
Volume148
Issue number1-2
DOIs
StatePublished - Jan 20 2012

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Stem cells
Macaca mulatta
Tissue
Embryonic Structures
Assays
Animals
Agglomeration
Cell Aggregation
Embryonic Stem Cells
Placenta
Primates
Stem Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tachibana, M., Sparman, M., Ramsey, C., Ma, H., Lee, H. S., Penedo, M. C. T., & Mitalipov, S. (2012). Generation of chimeric rhesus monkeys. Cell, 148(1-2), 285-295. https://doi.org/10.1016/j.cell.2011.12.007

Generation of chimeric rhesus monkeys. / Tachibana, Masahito; Sparman, Michelle; Ramsey, Cathy; Ma, Hong; Lee, Hyo Sang; Penedo, Maria Cecilia T; Mitalipov, Shoukhrat.

In: Cell, Vol. 148, No. 1-2, 20.01.2012, p. 285-295.

Research output: Contribution to journalArticle

Tachibana, M, Sparman, M, Ramsey, C, Ma, H, Lee, HS, Penedo, MCT & Mitalipov, S 2012, 'Generation of chimeric rhesus monkeys', Cell, vol. 148, no. 1-2, pp. 285-295. https://doi.org/10.1016/j.cell.2011.12.007
Tachibana M, Sparman M, Ramsey C, Ma H, Lee HS, Penedo MCT et al. Generation of chimeric rhesus monkeys. Cell. 2012 Jan 20;148(1-2):285-295. https://doi.org/10.1016/j.cell.2011.12.007
Tachibana, Masahito ; Sparman, Michelle ; Ramsey, Cathy ; Ma, Hong ; Lee, Hyo Sang ; Penedo, Maria Cecilia T ; Mitalipov, Shoukhrat. / Generation of chimeric rhesus monkeys. In: Cell. 2012 ; Vol. 148, No. 1-2. pp. 285-295.
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