Generation of a life-expanded rhesus monkey fibroblast cell line for the growth of rhesus rhadinovirus (RRV)

V. Kirchoff, Scott Wong, S. St.Jeor, G. S. Pari

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

RRV, the rhesus macaque equivalent to HHV-8 or kaposi's sarcoma-associated herpesvirus (KSHV) was recently isolated from a simian immunodeficiency virus (SIV) infected macaque with a lymphoproliferative disorder. The growth of RRV in tissue culture requires propagation of primary rhesus monkey fibroblasts (RFs). In an effort to extend the life of these primary cells in tissue culture, the catalytic subunit of telomerase (hTERT) was introduced into RF cells using a recombinant retrovirus. This new cell line, Telo-RFs, have currently been passed in tissue culture over 80 times compared to a maximum passage number of 38 for wild type RFs, remain fully permissive for RRV DNA replication and production of infectious virus. Viral gene expression of immediate-early and early RNA transcripts was virtually identical to that observed in wild-type (wt) RFs. In addition, transfection experiments show that telo-RFs are easily and more efficiently transfected than wtRFs.

Original languageEnglish (US)
Pages (from-to)321-333
Number of pages13
JournalArchives of Virology
Volume147
Issue number2
DOIs
StatePublished - 2002

Fingerprint

Rhadinovirus
Macaca mulatta
Fibroblasts
Cell Line
Growth
Human Herpesvirus 8
Simian Immunodeficiency Virus
Lymphoproliferative Disorders
Viral Genes
Telomerase
Macaca
Retroviridae
DNA Replication
Transfection
RNA
Viruses
Gene Expression

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Generation of a life-expanded rhesus monkey fibroblast cell line for the growth of rhesus rhadinovirus (RRV). / Kirchoff, V.; Wong, Scott; St.Jeor, S.; Pari, G. S.

In: Archives of Virology, Vol. 147, No. 2, 2002, p. 321-333.

Research output: Contribution to journalArticle

@article{45b6aa586cb04290a75dcc3179cef3db,
title = "Generation of a life-expanded rhesus monkey fibroblast cell line for the growth of rhesus rhadinovirus (RRV)",
abstract = "RRV, the rhesus macaque equivalent to HHV-8 or kaposi's sarcoma-associated herpesvirus (KSHV) was recently isolated from a simian immunodeficiency virus (SIV) infected macaque with a lymphoproliferative disorder. The growth of RRV in tissue culture requires propagation of primary rhesus monkey fibroblasts (RFs). In an effort to extend the life of these primary cells in tissue culture, the catalytic subunit of telomerase (hTERT) was introduced into RF cells using a recombinant retrovirus. This new cell line, Telo-RFs, have currently been passed in tissue culture over 80 times compared to a maximum passage number of 38 for wild type RFs, remain fully permissive for RRV DNA replication and production of infectious virus. Viral gene expression of immediate-early and early RNA transcripts was virtually identical to that observed in wild-type (wt) RFs. In addition, transfection experiments show that telo-RFs are easily and more efficiently transfected than wtRFs.",
author = "V. Kirchoff and Scott Wong and S. St.Jeor and Pari, {G. S.}",
year = "2002",
doi = "10.1007/s705-002-8322-9",
language = "English (US)",
volume = "147",
pages = "321--333",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "2",

}

TY - JOUR

T1 - Generation of a life-expanded rhesus monkey fibroblast cell line for the growth of rhesus rhadinovirus (RRV)

AU - Kirchoff, V.

AU - Wong, Scott

AU - St.Jeor, S.

AU - Pari, G. S.

PY - 2002

Y1 - 2002

N2 - RRV, the rhesus macaque equivalent to HHV-8 or kaposi's sarcoma-associated herpesvirus (KSHV) was recently isolated from a simian immunodeficiency virus (SIV) infected macaque with a lymphoproliferative disorder. The growth of RRV in tissue culture requires propagation of primary rhesus monkey fibroblasts (RFs). In an effort to extend the life of these primary cells in tissue culture, the catalytic subunit of telomerase (hTERT) was introduced into RF cells using a recombinant retrovirus. This new cell line, Telo-RFs, have currently been passed in tissue culture over 80 times compared to a maximum passage number of 38 for wild type RFs, remain fully permissive for RRV DNA replication and production of infectious virus. Viral gene expression of immediate-early and early RNA transcripts was virtually identical to that observed in wild-type (wt) RFs. In addition, transfection experiments show that telo-RFs are easily and more efficiently transfected than wtRFs.

AB - RRV, the rhesus macaque equivalent to HHV-8 or kaposi's sarcoma-associated herpesvirus (KSHV) was recently isolated from a simian immunodeficiency virus (SIV) infected macaque with a lymphoproliferative disorder. The growth of RRV in tissue culture requires propagation of primary rhesus monkey fibroblasts (RFs). In an effort to extend the life of these primary cells in tissue culture, the catalytic subunit of telomerase (hTERT) was introduced into RF cells using a recombinant retrovirus. This new cell line, Telo-RFs, have currently been passed in tissue culture over 80 times compared to a maximum passage number of 38 for wild type RFs, remain fully permissive for RRV DNA replication and production of infectious virus. Viral gene expression of immediate-early and early RNA transcripts was virtually identical to that observed in wild-type (wt) RFs. In addition, transfection experiments show that telo-RFs are easily and more efficiently transfected than wtRFs.

UR - http://www.scopus.com/inward/record.url?scp=0036008462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036008462&partnerID=8YFLogxK

U2 - 10.1007/s705-002-8322-9

DO - 10.1007/s705-002-8322-9

M3 - Article

VL - 147

SP - 321

EP - 333

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 2

ER -