General utility of the chicken βB1-crystallin promoter to drive protein expression in lens fiber cells of transgenic mice

Jennifer R. Taube, Chun Y. Gao, Yoji Ueda, Peggy S. Zelenka, Larry David, Melinda K. Duncan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Transgenic mouse technology has been very valuable for the study of lens fiber cells since they can not be propagated in cell culture. The targeting of transgenes to the lens has traditionally been done with the αA-crystallin promoter. However, while lens-specific, transgenic lines made with the αA-crystallin promoter express the transgene at levels 100-300-fold lower than endogenous αA-crystallin. Here we propose an alternative, the chicken βB1-crystallin promoter (-432/+30). Transgenic mice made with this promoter have successfully expressed CAT, d/n m-calpain, Wee 1, and βB2-crystallin mRNA at levels comparable to the endogenous βB1-crystallin gene and no eye abnormalities such as cataracts, have resulted. All of the transgenic lines made with the chicken βB1-crystallin promoter have expressed the transgene in the lens fiber cells, and the best lines express at levels close to endogenous βB1-crystallin. While RNA expression is very high, only moderate protein expression has been achieved, implying that the high protein expression of the crystallins is partially controlled at the level of translation. Thus, the chicken βB1-crystallin promoter directs high level RNA expression to lens fiber cells, which may be especially useful for the expression of ribozyme and anti-sense RNAs in addition to ectopic proteins.

Original languageEnglish (US)
Pages (from-to)397-410
Number of pages14
JournalTransgenic Research
Volume11
Issue number4
DOIs
StatePublished - Aug 2002

Fingerprint

crystallins
fiber cells
Crystallins
Lens
Transgenic Mice
Lenses
Chickens
protein synthesis
promoter regions
genetically modified organisms
chickens
mice
Proteins
Transgenes
transgenes
Eye Abnormalities
RNA
antisense RNA
Antisense RNA
Catalytic RNA

Keywords

  • βB1-crystallin
  • Cataract
  • Eye
  • Gene expression
  • Lens
  • Transgenic mice

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology
  • Food Science

Cite this

General utility of the chicken βB1-crystallin promoter to drive protein expression in lens fiber cells of transgenic mice. / Taube, Jennifer R.; Gao, Chun Y.; Ueda, Yoji; Zelenka, Peggy S.; David, Larry; Duncan, Melinda K.

In: Transgenic Research, Vol. 11, No. 4, 08.2002, p. 397-410.

Research output: Contribution to journalArticle

Taube, Jennifer R. ; Gao, Chun Y. ; Ueda, Yoji ; Zelenka, Peggy S. ; David, Larry ; Duncan, Melinda K. / General utility of the chicken βB1-crystallin promoter to drive protein expression in lens fiber cells of transgenic mice. In: Transgenic Research. 2002 ; Vol. 11, No. 4. pp. 397-410.
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