General considerations for live imaging of developing hippocampal neurons in culture

Stefanie Kaech, Chun Fang Huang, Gary Banker

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Dissociated cell cultures of the rodent hippocampus have become a standard model for studying many facets of neural development, including the development of polarity, axonal and dendritic growth, and synapse formation. The cultures are quite homogeneous-~90% of the cells are pyramidal neurons- and it is relatively easy to express green fluorescent protein (GFP)-tagged proteins by transfection. This article describes the cultures and the key features of the system used to image them. It also includes suggestions on labeling cells with GFP-tagged proteins. It concludes with a discussion of the advantages and disadvantages of this culture system.

Original languageEnglish (US)
Pages (from-to)312-318
Number of pages7
JournalCold Spring Harbor Protocols
Volume7
Issue number3
DOIs
StatePublished - Mar 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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