General considerations for live imaging of developing hippocampal neurons in culture

Stefanie Kaech-Petrie, Chun Fang Huang, Gary Banker

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Dissociated cell cultures of the rodent hippocampus have become a standard model for studying many facets of neural development, including the development of polarity, axonal and dendritic growth, and synapse formation. The cultures are quite homogeneous-~90% of the cells are pyramidal neurons- and it is relatively easy to express green fluorescent protein (GFP)-tagged proteins by transfection. This article describes the cultures and the key features of the system used to image them. It also includes suggestions on labeling cells with GFP-tagged proteins. It concludes with a discussion of the advantages and disadvantages of this culture system.

Original languageEnglish (US)
Pages (from-to)312-318
Number of pages7
JournalCold Spring Harbor Protocols
Volume7
Issue number3
DOIs
StatePublished - Mar 2012
Externally publishedYes

Fingerprint

Green Fluorescent Proteins
Cell culture
Neurons
Body Patterning
Imaging techniques
Pyramidal Cells
Synapses
Labeling
Transfection
Rodentia
Hippocampus
Proteins
Cell Culture Techniques
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

General considerations for live imaging of developing hippocampal neurons in culture. / Kaech-Petrie, Stefanie; Huang, Chun Fang; Banker, Gary.

In: Cold Spring Harbor Protocols, Vol. 7, No. 3, 03.2012, p. 312-318.

Research output: Contribution to journalArticle

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