Gene expression profiling of the donor kidney at the time of transplantation predicts clinical outcomes 2 years after transplantation

Gabor Bodonyi-Kovacs, Prabhakar Putheti, Miguel Marino, Yingyos Avihingsanon, Marc E. Uknis, Anthony P. Monaco, Terry B. Strom, Martha Pavlakis

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We have previously demonstrated that biomarkers of inflammation and immune activity detected within intraoperative renal transplant allograft biopsies are linked to adverse short-term post-transplantation clinical outcomes. Now we provide a post hoc analysis of our earlier data in the light of longer clinical follow-up. A total of 75 consecutively performed renal allografts were analyzed for gene expression of proinflammatory molecules, inflammation-induced adhesion molecules, and antiapoptotic genes expressed 15 minutes after vascular reperfusion to determine whether this analysis can aid in predicting long-term quality of renal function, proteinuria, graft loss, and death-censored graft. We have built predictive models for proteinuria (area under the curve = 0.859, p = 0.0001) and graft loss (area under the curve = 0.724, p = 0.027) 2 years post-transplantation using clinical variables in combination with intragraft gene expression data of tumor necrosis factor-α, interleukin-6, CD40, CD3, and tumor necrosis factor-α, Bcl-2, and interferon-γ, respectively. This post hoc analysis demonstrates that hypothesis-driven, targeted polymerase chain reaction profiling of gene expression in the donor kidney at the time of engraftment can predict 2-year post-transplantation clinical outcomes.

Original languageEnglish (US)
Pages (from-to)451-455
Number of pages5
JournalHuman Immunology
Volume71
Issue number5
DOIs
StatePublished - May 1 2010
Externally publishedYes

Keywords

  • Graft loss
  • PCR
  • Prediction
  • Proteinuria
  • Renal transplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Bodonyi-Kovacs, G., Putheti, P., Marino, M., Avihingsanon, Y., Uknis, M. E., Monaco, A. P., Strom, T. B., & Pavlakis, M. (2010). Gene expression profiling of the donor kidney at the time of transplantation predicts clinical outcomes 2 years after transplantation. Human Immunology, 71(5), 451-455. https://doi.org/10.1016/j.humimm.2010.02.013