Gene discovery by microarray

Identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation

David Kuninger, Ryan Kuzmickas, Bonnie Peng, John E. Pintar, Peter Rotwein

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Peptide growth factors regulate cell fate by activating distinct signal transduction pathways that ultimately influence gene expression. Insulin-like growth factors (IGFs) play central roles in controlling somatic growth and participate in skeletal muscle development and regeneration. In cultured muscle cells, IGF action is critical both for maintaining viability during the transition from proliferating to differentiating myoblasts and for facilitating differentiation. By contrast, platelet-derived growth factor (PDGF) can sustain cell survival but inhibits differentiation. Here we examine the genetic programs that accompany IGF and PDGF action in myoblasts. Through analysis of high-density oligonucleotide arrays containing ∼36,000 mouse probe sets, we identify 90 transcripts differentially induced by IGF-I, including 28 muscle-specific genes and 33 previously unannotated mRNAs, and 55 transcripts specifically stimulated by PDGF, including 14 unknowns. Detailed study of one IGF-induced mRNA shows that it encodes a protein related to a recently characterized repulsive guidance molecule postulated to regulate neuronal targeting during development. Our results demonstrate the power of transcriptional profiling for gene discovery and provide opportunities for investigating new proteins potentially involved in different aspects of growth factor action in muscle.

Original languageEnglish (US)
Pages (from-to)876-889
Number of pages14
JournalGenomics
Volume84
Issue number5
DOIs
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Genetic Association Studies
Somatomedins
Muscle Cells
Cell Differentiation
Platelet-Derived Growth Factor
Cell Survival
Intercellular Signaling Peptides and Proteins
Myoblasts
Genes
Muscles
Messenger RNA
Muscle Development
Oligonucleotide Array Sequence Analysis
Insulin-Like Growth Factor I
Regeneration
Cultured Cells
Signal Transduction
Skeletal Muscle
Proteins
Gene Expression

Keywords

  • IGF-I
  • IGF-II
  • Muscle cells
  • Myoblasts
  • Oligonucleotide arrays
  • PDGF
  • RGMc

ASJC Scopus subject areas

  • Genetics

Cite this

Gene discovery by microarray : Identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation. / Kuninger, David; Kuzmickas, Ryan; Peng, Bonnie; Pintar, John E.; Rotwein, Peter.

In: Genomics, Vol. 84, No. 5, 11.2004, p. 876-889.

Research output: Contribution to journalArticle

Kuninger, David ; Kuzmickas, Ryan ; Peng, Bonnie ; Pintar, John E. ; Rotwein, Peter. / Gene discovery by microarray : Identification of novel genes induced during growth factor-mediated muscle cell survival and differentiation. In: Genomics. 2004 ; Vol. 84, No. 5. pp. 876-889.
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