Gene delivery to human chondrocytes by an adeno associated virus vector

Yuji Arai, Toshikazu Kubo, Shinji Fushiki, Osam Mazda, Hiroyuki Nakai, Yuichi Iwaki, Jiro Imanishi, Yasusuke Hirasawa

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Objective. To investigate the efficiency of gene transduction to human chondrocytes using an adeno associated virus (AAV) vector. Methods. We transduced green fluorescent protein (GFP) gene using AAV vector to primary human chondrocytes as well as human cartilage organ cultures, in which chondrocytes are surrounded by extracellular matrix. Expression of GFP gene was analyzed at various time points after transduction by fluorescence microscopy and immunohistochemistry. Results. In primary chondrocytes, the percentages of GFP positive cells were 15.9% or 16.0% on Day 1 and 95.0% or 93.7% on Day 7 after gene transduction. In cartilage organ cultures, gene delivery was observed in cells located not only in the superficial layer but also in the deep layer within the cartilage tissue. Up to 45.3 ± 7.4% or 46.0 ± 3.9% of chondrocytes expressed GFP for at least 28 days. Conclusion. AAV vector could be useful for direct gene delivery to chondrocytes in situ.

Original languageEnglish (US)
Pages (from-to)979-982
Number of pages4
JournalJournal of Rheumatology
Volume27
Issue number4
StatePublished - Jun 30 2000

Keywords

  • Adeno associated virus vector
  • Cartilage
  • Gene therapy
  • Osteoarthritis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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    Arai, Y., Kubo, T., Fushiki, S., Mazda, O., Nakai, H., Iwaki, Y., Imanishi, J., & Hirasawa, Y. (2000). Gene delivery to human chondrocytes by an adeno associated virus vector. Journal of Rheumatology, 27(4), 979-982.