Gdnf Activates Midline Repulsion by Semaphorin3B via NCAM during Commissural Axon Guidance

Camille Charoy, Homaira Nawabi, Florie Reynaud, Edmund Derrington, Muriel Bozon, Kevin Wright, Julien Falk, Françoise Helmbacher, Karine Kindbeiter, Valérie Castellani

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The Neurotrophic factor gdnf plays diverse developmental roles, supporting survival and also acting as a chemoattractant for axon and cell migration. We report that in the developing spinal cord, a focal source of gdnf is present in the floor plate (FP) where commissural axons cross the midline. Gdnf has no direct guidance properties but switches on the responsiveness of crossing commissural growth cones to the midline repellent Semaphorin3B by suppressing calpain-mediated processing of the Sema3B signaling coreceptor Plexin-A1. Analysis of single and double mutant mouse models indicates that although gdnf is the principal trigger of Sema3B midline repulsion, it acts with another FP cue, NrCAM. Finally, genetic and in vitro experiments provide evidence that this gdnf effect is RET independent and mediated by NCAM/GFRα1 signaling. This study identifies a regulator of midline crossing and reveals interplays between Semaphorin and gdnf signaling during axon guidance.

Original languageEnglish (US)
Pages (from-to)1051-1066
Number of pages16
JournalNeuron
Volume75
Issue number6
DOIs
StatePublished - Sep 20 2012
Externally publishedYes

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Neural Cell Adhesion Molecules
Axons
Semaphorins
Growth Cones
Calpain
Chemotactic Factors
Nerve Growth Factors
Cell Movement
Cues
Spinal Cord
Axon Guidance
In Vitro Techniques
plexin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Charoy, C., Nawabi, H., Reynaud, F., Derrington, E., Bozon, M., Wright, K., ... Castellani, V. (2012). Gdnf Activates Midline Repulsion by Semaphorin3B via NCAM during Commissural Axon Guidance. Neuron, 75(6), 1051-1066. https://doi.org/10.1016/j.neuron.2012.08.021

Gdnf Activates Midline Repulsion by Semaphorin3B via NCAM during Commissural Axon Guidance. / Charoy, Camille; Nawabi, Homaira; Reynaud, Florie; Derrington, Edmund; Bozon, Muriel; Wright, Kevin; Falk, Julien; Helmbacher, Françoise; Kindbeiter, Karine; Castellani, Valérie.

In: Neuron, Vol. 75, No. 6, 20.09.2012, p. 1051-1066.

Research output: Contribution to journalArticle

Charoy, C, Nawabi, H, Reynaud, F, Derrington, E, Bozon, M, Wright, K, Falk, J, Helmbacher, F, Kindbeiter, K & Castellani, V 2012, 'Gdnf Activates Midline Repulsion by Semaphorin3B via NCAM during Commissural Axon Guidance', Neuron, vol. 75, no. 6, pp. 1051-1066. https://doi.org/10.1016/j.neuron.2012.08.021
Charoy, Camille ; Nawabi, Homaira ; Reynaud, Florie ; Derrington, Edmund ; Bozon, Muriel ; Wright, Kevin ; Falk, Julien ; Helmbacher, Françoise ; Kindbeiter, Karine ; Castellani, Valérie. / Gdnf Activates Midline Repulsion by Semaphorin3B via NCAM during Commissural Axon Guidance. In: Neuron. 2012 ; Vol. 75, No. 6. pp. 1051-1066.
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