Gating properties of single SK channels in hippocampal CA1 pyramidal neurons

Birgit Hirschberg, James Maylie, John Adelman, Neil V. Marrion

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The activation of small-conductance calcium-activated potassium channels (SK) has a profound effect on membrane excitability. In hippocampal pyramidal neurons, SK channel activation by Ca2+ entry from a preceding burst of action potentials generates the slow afterhyperpolarization (AHP). Stimulation of a number of receptor types suppresses the slow AHP, inhibiting spike frequency adaptation and causing these neurons to fire tonically. Little is known of the gating properties of native SK channels in CNS neurons. By using excised inside-out patches, a small-amplitude channel has been resolved that was half-activated by ~0.6 μM Ca2+ in a voltage- independent manner. The channel possessed a slope conductance of 10 pS and exhibited nonstationary gating. These properties are in accord with those of cloned SK channels. The measured Ca2+ sensitivity of hippocampal SK channels suggests that the slow AHP is generated by activation of SK channels from a local rise of intracellular Ca2+.

Original languageEnglish (US)
Pages (from-to)1905-1913
Number of pages9
JournalBiophysical Journal
Volume77
Issue number4
StatePublished - Oct 1999

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Pyramidal Cells
Small-Conductance Calcium-Activated Potassium Channels
Neurons
Action Potentials
Membranes

ASJC Scopus subject areas

  • Biophysics

Cite this

Hirschberg, B., Maylie, J., Adelman, J., & Marrion, N. V. (1999). Gating properties of single SK channels in hippocampal CA1 pyramidal neurons. Biophysical Journal, 77(4), 1905-1913.

Gating properties of single SK channels in hippocampal CA1 pyramidal neurons. / Hirschberg, Birgit; Maylie, James; Adelman, John; Marrion, Neil V.

In: Biophysical Journal, Vol. 77, No. 4, 10.1999, p. 1905-1913.

Research output: Contribution to journalArticle

Hirschberg, B, Maylie, J, Adelman, J & Marrion, NV 1999, 'Gating properties of single SK channels in hippocampal CA1 pyramidal neurons', Biophysical Journal, vol. 77, no. 4, pp. 1905-1913.
Hirschberg, Birgit ; Maylie, James ; Adelman, John ; Marrion, Neil V. / Gating properties of single SK channels in hippocampal CA1 pyramidal neurons. In: Biophysical Journal. 1999 ; Vol. 77, No. 4. pp. 1905-1913.
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