GATA transcription factor required for immunity to bacterial and fungal pathogens

Samantha Kerry, Michael TeKippe, Nathan C. Gaddis, Alejandro Aballay

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In the past decade, Caenorhabditis elegans has been used to dissect several genetic pathways involved in immunity; however, little is known about transcription factors that regulate the expression of immune effectors. C. elegans does not appear to have a functional homolog of the key immune transcription factor NF-κB, Here we show that that the intestinal GATA transcription factor ELT-2 is required for both immunity to Salmonella enterica and expression of a C-type lectin gene, dec-67, which is expressed in the intestinal cells and is a good marker of S. enterica infection. We also found that ELT-2 is required for immunity to Pseudomonas aeruginosa, Enterococcus faecalis, and Cryptococcus neoformans. Lack of immune inhibition by DAF-2, which negatively regulates the FOXO transcription factor DAF-16, rescues the hypersusceptibility to pathogens phenotype of elt-2(RNAi) animals. Our results indicate that ELT-2 is part of a multi-pathogen defense pathway that regulates innate immunity independently of the DAF-2/DAF-16 signaling pathway.

Original languageEnglish (US)
Article numbere77
JournalPLoS One
Volume1
Issue number1
DOIs
StatePublished - Dec 20 2006
Externally publishedYes

Fingerprint

GATA Transcription Factors
Pathogens
Immunity
Transcription Factors
Salmonella enterica
transcription factors
immunity
Caenorhabditis elegans
pathogens
GATA2 Transcription Factor
C-Type Lectins
Cryptococcus neoformans
Salmonella
Salmonella Infections
Enterococcus faecalis
Immunologic Factors
RNA Interference
Pseudomonas aeruginosa
Innate Immunity
lectins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

GATA transcription factor required for immunity to bacterial and fungal pathogens. / Kerry, Samantha; TeKippe, Michael; Gaddis, Nathan C.; Aballay, Alejandro.

In: PLoS One, Vol. 1, No. 1, e77, 20.12.2006.

Research output: Contribution to journalArticle

Kerry, Samantha ; TeKippe, Michael ; Gaddis, Nathan C. ; Aballay, Alejandro. / GATA transcription factor required for immunity to bacterial and fungal pathogens. In: PLoS One. 2006 ; Vol. 1, No. 1.
@article{f78e493090f7476d98a5ed92bdd58a2e,
title = "GATA transcription factor required for immunity to bacterial and fungal pathogens",
abstract = "In the past decade, Caenorhabditis elegans has been used to dissect several genetic pathways involved in immunity; however, little is known about transcription factors that regulate the expression of immune effectors. C. elegans does not appear to have a functional homolog of the key immune transcription factor NF-κB, Here we show that that the intestinal GATA transcription factor ELT-2 is required for both immunity to Salmonella enterica and expression of a C-type lectin gene, dec-67, which is expressed in the intestinal cells and is a good marker of S. enterica infection. We also found that ELT-2 is required for immunity to Pseudomonas aeruginosa, Enterococcus faecalis, and Cryptococcus neoformans. Lack of immune inhibition by DAF-2, which negatively regulates the FOXO transcription factor DAF-16, rescues the hypersusceptibility to pathogens phenotype of elt-2(RNAi) animals. Our results indicate that ELT-2 is part of a multi-pathogen defense pathway that regulates innate immunity independently of the DAF-2/DAF-16 signaling pathway.",
author = "Samantha Kerry and Michael TeKippe and Gaddis, {Nathan C.} and Alejandro Aballay",
year = "2006",
month = "12",
day = "20",
doi = "10.1371/journal.pone.0000077",
language = "English (US)",
volume = "1",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

TY - JOUR

T1 - GATA transcription factor required for immunity to bacterial and fungal pathogens

AU - Kerry, Samantha

AU - TeKippe, Michael

AU - Gaddis, Nathan C.

AU - Aballay, Alejandro

PY - 2006/12/20

Y1 - 2006/12/20

N2 - In the past decade, Caenorhabditis elegans has been used to dissect several genetic pathways involved in immunity; however, little is known about transcription factors that regulate the expression of immune effectors. C. elegans does not appear to have a functional homolog of the key immune transcription factor NF-κB, Here we show that that the intestinal GATA transcription factor ELT-2 is required for both immunity to Salmonella enterica and expression of a C-type lectin gene, dec-67, which is expressed in the intestinal cells and is a good marker of S. enterica infection. We also found that ELT-2 is required for immunity to Pseudomonas aeruginosa, Enterococcus faecalis, and Cryptococcus neoformans. Lack of immune inhibition by DAF-2, which negatively regulates the FOXO transcription factor DAF-16, rescues the hypersusceptibility to pathogens phenotype of elt-2(RNAi) animals. Our results indicate that ELT-2 is part of a multi-pathogen defense pathway that regulates innate immunity independently of the DAF-2/DAF-16 signaling pathway.

AB - In the past decade, Caenorhabditis elegans has been used to dissect several genetic pathways involved in immunity; however, little is known about transcription factors that regulate the expression of immune effectors. C. elegans does not appear to have a functional homolog of the key immune transcription factor NF-κB, Here we show that that the intestinal GATA transcription factor ELT-2 is required for both immunity to Salmonella enterica and expression of a C-type lectin gene, dec-67, which is expressed in the intestinal cells and is a good marker of S. enterica infection. We also found that ELT-2 is required for immunity to Pseudomonas aeruginosa, Enterococcus faecalis, and Cryptococcus neoformans. Lack of immune inhibition by DAF-2, which negatively regulates the FOXO transcription factor DAF-16, rescues the hypersusceptibility to pathogens phenotype of elt-2(RNAi) animals. Our results indicate that ELT-2 is part of a multi-pathogen defense pathway that regulates innate immunity independently of the DAF-2/DAF-16 signaling pathway.

UR - http://www.scopus.com/inward/record.url?scp=54949117914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54949117914&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0000077

DO - 10.1371/journal.pone.0000077

M3 - Article

C2 - 17183709

AN - SCOPUS:54949117914

VL - 1

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 1

M1 - e77

ER -