Gastrointestinal absorption of epidermal growth factor in suckling rats

W. Thornburg, L. Matrisian, B. Magun, O. Koldovsky

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) was detected in the milk of adult lactating Sprague-Dawley female rats (38.85 ng/ml) and in the stomach (37.25 ng/g content) and plasma (32.36 ng/ml) of 13-day-old suckling offspring. Sixty-nanogram (0.12 mCi/ml) doses of 125I-EGF were administered orally to sucklings in 200 μl of buffer for 0, 0.5, 1.0, and 3.0 h. Lung, liver, kidney, brain, and blood each contained 1% or less of the administered radioactivity. Stomach wall (8%) and content (63%), intestinal wall (15%) and flushing (38%), and skin (18%) contained larger amounts reaching maxima in these three regions at 0, 0.5, and 3.0 h, respectively. Except for skin, a substantial amount of radioactivity from all tissues represented intact (6-90%) and immunoreactive (3-90%) EGF based on Sephadex G-25 chromatography and anti-EGF antibody binding, respectively. From 30 to 55% of the radioactivity from wall (gastric or small intestinal) or lumina was also capable of binding to A-431 cell surface receptors. Isoelectric points of major species found in stomach (4.2), intestine (4.1), and other tissues differed from that of administered EGF (4.5).

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume9
Issue number1
StatePublished - 1984
Externally publishedYes

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Epidermal Growth Factor
Stomach
Radioactivity
Skin
Gastrointestinal Contents
Isoelectric Point
Cell Surface Receptors
Intestines
Sprague Dawley Rats
Chromatography
Buffers
Milk
Gastrointestinal Absorption
Kidney
Lung
Antibodies
Liver
Brain

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Gastrointestinal absorption of epidermal growth factor in suckling rats. / Thornburg, W.; Matrisian, L.; Magun, B.; Koldovsky, O.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 9, No. 1, 1984.

Research output: Contribution to journalArticle

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