Gastrointestinal absorption of epidermal growth factor in suckling rats.

W. Thornburg, L. Matrisian, B. Magun, O. Koldovský

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) was detected in the milk of adult lactating Sprague-Dawley female rats (38.85 ng/ml) and in the stomach (37.25 ng/g content) and plasma (32.36 ng/ml) of 13-day-old suckling offspring. Sixty-nanogram (0.12 mCi/ml) doses of 125I-EGF were administered orally to sucklings in 200 microliters of buffer for 0, 0.5, 1.0, and 3.0 h. Lung, liver kidney, brain, and blood each contained 1% or less of the administered radioactivity. Stomach wall (8%) and content (63%), intestinal wall (15%) and flushing (38%), and skin (18%) contained larger amounts reaching maxima in these three regions at 0, 0.5, and 3.0 h, respectively. Except for skin, a substantial amount of radioactivity from all tissues represented intact (6-90%) and immunoreactive (3-90%) EGF based on Sephadex G-25 chromatography and anti-EGF antibody binding, respectively. From 30 to 55% of the radioactivity from wall (gastric or small intestinal) or lumina was also capable of binding to A-431 cell surface receptors. Isoelectric points of major species found in stomach (4.2), intestine (4.1), and other tissues differed from that of administered EGF (4.5).

Original languageEnglish (US)
JournalThe American journal of physiology
Volume246
Issue number1 Pt 1
StatePublished - Jan 1984

Fingerprint

Epidermal Growth Factor
Stomach
Radioactivity
Skin
Gastrointestinal Contents
Isoelectric Point
Cell Surface Receptors
Intestines
Sprague Dawley Rats
Chromatography
Buffers
Milk
Gastrointestinal Absorption
Kidney
Lung
Antibodies
Liver
Brain

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Thornburg, W., Matrisian, L., Magun, B., & Koldovský, O. (1984). Gastrointestinal absorption of epidermal growth factor in suckling rats. The American journal of physiology, 246(1 Pt 1).

Gastrointestinal absorption of epidermal growth factor in suckling rats. / Thornburg, W.; Matrisian, L.; Magun, B.; Koldovský, O.

In: The American journal of physiology, Vol. 246, No. 1 Pt 1, 01.1984.

Research output: Contribution to journalArticle

Thornburg, W, Matrisian, L, Magun, B & Koldovský, O 1984, 'Gastrointestinal absorption of epidermal growth factor in suckling rats.', The American journal of physiology, vol. 246, no. 1 Pt 1.
Thornburg W, Matrisian L, Magun B, Koldovský O. Gastrointestinal absorption of epidermal growth factor in suckling rats. The American journal of physiology. 1984 Jan;246(1 Pt 1).
Thornburg, W. ; Matrisian, L. ; Magun, B. ; Koldovský, O. / Gastrointestinal absorption of epidermal growth factor in suckling rats. In: The American journal of physiology. 1984 ; Vol. 246, No. 1 Pt 1.
@article{767124298f6e482db82dd4c2d5cc777f,
title = "Gastrointestinal absorption of epidermal growth factor in suckling rats.",
abstract = "Epidermal growth factor (EGF) was detected in the milk of adult lactating Sprague-Dawley female rats (38.85 ng/ml) and in the stomach (37.25 ng/g content) and plasma (32.36 ng/ml) of 13-day-old suckling offspring. Sixty-nanogram (0.12 mCi/ml) doses of 125I-EGF were administered orally to sucklings in 200 microliters of buffer for 0, 0.5, 1.0, and 3.0 h. Lung, liver kidney, brain, and blood each contained 1{\%} or less of the administered radioactivity. Stomach wall (8{\%}) and content (63{\%}), intestinal wall (15{\%}) and flushing (38{\%}), and skin (18{\%}) contained larger amounts reaching maxima in these three regions at 0, 0.5, and 3.0 h, respectively. Except for skin, a substantial amount of radioactivity from all tissues represented intact (6-90{\%}) and immunoreactive (3-90{\%}) EGF based on Sephadex G-25 chromatography and anti-EGF antibody binding, respectively. From 30 to 55{\%} of the radioactivity from wall (gastric or small intestinal) or lumina was also capable of binding to A-431 cell surface receptors. Isoelectric points of major species found in stomach (4.2), intestine (4.1), and other tissues differed from that of administered EGF (4.5).",
author = "W. Thornburg and L. Matrisian and B. Magun and O. Koldovsk{\'y}",
year = "1984",
month = "1",
language = "English (US)",
volume = "246",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "1 Pt 1",

}

TY - JOUR

T1 - Gastrointestinal absorption of epidermal growth factor in suckling rats.

AU - Thornburg, W.

AU - Matrisian, L.

AU - Magun, B.

AU - Koldovský, O.

PY - 1984/1

Y1 - 1984/1

N2 - Epidermal growth factor (EGF) was detected in the milk of adult lactating Sprague-Dawley female rats (38.85 ng/ml) and in the stomach (37.25 ng/g content) and plasma (32.36 ng/ml) of 13-day-old suckling offspring. Sixty-nanogram (0.12 mCi/ml) doses of 125I-EGF were administered orally to sucklings in 200 microliters of buffer for 0, 0.5, 1.0, and 3.0 h. Lung, liver kidney, brain, and blood each contained 1% or less of the administered radioactivity. Stomach wall (8%) and content (63%), intestinal wall (15%) and flushing (38%), and skin (18%) contained larger amounts reaching maxima in these three regions at 0, 0.5, and 3.0 h, respectively. Except for skin, a substantial amount of radioactivity from all tissues represented intact (6-90%) and immunoreactive (3-90%) EGF based on Sephadex G-25 chromatography and anti-EGF antibody binding, respectively. From 30 to 55% of the radioactivity from wall (gastric or small intestinal) or lumina was also capable of binding to A-431 cell surface receptors. Isoelectric points of major species found in stomach (4.2), intestine (4.1), and other tissues differed from that of administered EGF (4.5).

AB - Epidermal growth factor (EGF) was detected in the milk of adult lactating Sprague-Dawley female rats (38.85 ng/ml) and in the stomach (37.25 ng/g content) and plasma (32.36 ng/ml) of 13-day-old suckling offspring. Sixty-nanogram (0.12 mCi/ml) doses of 125I-EGF were administered orally to sucklings in 200 microliters of buffer for 0, 0.5, 1.0, and 3.0 h. Lung, liver kidney, brain, and blood each contained 1% or less of the administered radioactivity. Stomach wall (8%) and content (63%), intestinal wall (15%) and flushing (38%), and skin (18%) contained larger amounts reaching maxima in these three regions at 0, 0.5, and 3.0 h, respectively. Except for skin, a substantial amount of radioactivity from all tissues represented intact (6-90%) and immunoreactive (3-90%) EGF based on Sephadex G-25 chromatography and anti-EGF antibody binding, respectively. From 30 to 55% of the radioactivity from wall (gastric or small intestinal) or lumina was also capable of binding to A-431 cell surface receptors. Isoelectric points of major species found in stomach (4.2), intestine (4.1), and other tissues differed from that of administered EGF (4.5).

UR - http://www.scopus.com/inward/record.url?scp=0021291127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021291127&partnerID=8YFLogxK

M3 - Article

C2 - 6607683

AN - SCOPUS:0021291127

VL - 246

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 1 Pt 1

ER -