Gastrin-releasing peptide-like immunoreactivity is present in human maternal and fetal placental membranes

Q. Xiao, X. Han, J. R G Challis, D. J. Hill, Eliot Spindel, C. J. Prasad, K. Akagi, T. J. McDonald

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Extracts of human term amnion, placenta, and chorion/decidual tissue (n = 5) contained gastrin-releasing peptide-like immunoreactivity (GRPLI) in amounts of 4.7 ± 2.9 (pmol/g wet wt; mean ± SEM), 3.6 ± 1.1 and 2.9 ± 1.5, respectively. Using C-terminally directed antisera and gel filtration chromatography and reverse-phase high-performance liquid chromatography (HPLC), each tissue contained molecular forms consistent with the presence of GRP1-27 and GRP18-27 but also contained larger amounts of two GRPLI peaks, which apparently are novel GRP-like peptides. In contrast, tissue extracts of human fetal lung contained only GRP1-27, GRP14-27, and GRP18-27. Using RT-PCR and specific GRP primers and probes, messenger RNA encoding for GRP was readily demonstrable from 6-weeks gestation throughout pregnancy to term in full-thickness membranes, placental villi, and decidua. Positive immunohistochemical staining for GRP occurred in extravillous trophoblasts in decidual septa and fetal membranes, cytotrophoblasts, syncytiotrophoblast, and certain stromal cells in placental villi and amniotic epithelium. GRPLI and GRP messenger RNA were present from the earliest dates examined (6-9 weeks) throughout pregnancy to term. Given the proven trophic nature of GRP and related peptides, these peptides may play important roles in maternal, placental, and fetal development during human pregnancy.

    Original languageEnglish (US)
    Pages (from-to)3766-3773
    Number of pages8
    JournalJournal of Clinical Endocrinology and Metabolism
    Volume81
    Issue number10
    DOIs
    StatePublished - 1996

    Fingerprint

    Gastrin-Releasing Peptide
    Extraembryonic Membranes
    Trophoblasts
    Mothers
    Membranes
    Chorionic Villi
    Pregnancy
    Peptides
    Tissue
    Messenger RNA
    Tissue Extracts
    High performance liquid chromatography
    Chromatography
    Chorion
    Decidua
    Placentation
    Amnion
    Immune Sera
    Reverse-Phase Chromatography
    Stromal Cells

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Gastrin-releasing peptide-like immunoreactivity is present in human maternal and fetal placental membranes. / Xiao, Q.; Han, X.; Challis, J. R G; Hill, D. J.; Spindel, Eliot; Prasad, C. J.; Akagi, K.; McDonald, T. J.

    In: Journal of Clinical Endocrinology and Metabolism, Vol. 81, No. 10, 1996, p. 3766-3773.

    Research output: Contribution to journalArticle

    Xiao, Q. ; Han, X. ; Challis, J. R G ; Hill, D. J. ; Spindel, Eliot ; Prasad, C. J. ; Akagi, K. ; McDonald, T. J. / Gastrin-releasing peptide-like immunoreactivity is present in human maternal and fetal placental membranes. In: Journal of Clinical Endocrinology and Metabolism. 1996 ; Vol. 81, No. 10. pp. 3766-3773.
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    abstract = "Extracts of human term amnion, placenta, and chorion/decidual tissue (n = 5) contained gastrin-releasing peptide-like immunoreactivity (GRPLI) in amounts of 4.7 ± 2.9 (pmol/g wet wt; mean ± SEM), 3.6 ± 1.1 and 2.9 ± 1.5, respectively. Using C-terminally directed antisera and gel filtration chromatography and reverse-phase high-performance liquid chromatography (HPLC), each tissue contained molecular forms consistent with the presence of GRP1-27 and GRP18-27 but also contained larger amounts of two GRPLI peaks, which apparently are novel GRP-like peptides. In contrast, tissue extracts of human fetal lung contained only GRP1-27, GRP14-27, and GRP18-27. Using RT-PCR and specific GRP primers and probes, messenger RNA encoding for GRP was readily demonstrable from 6-weeks gestation throughout pregnancy to term in full-thickness membranes, placental villi, and decidua. Positive immunohistochemical staining for GRP occurred in extravillous trophoblasts in decidual septa and fetal membranes, cytotrophoblasts, syncytiotrophoblast, and certain stromal cells in placental villi and amniotic epithelium. GRPLI and GRP messenger RNA were present from the earliest dates examined (6-9 weeks) throughout pregnancy to term. Given the proven trophic nature of GRP and related peptides, these peptides may play important roles in maternal, placental, and fetal development during human pregnancy.",
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    AU - Challis, J. R G

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    AU - Prasad, C. J.

    AU - Akagi, K.

    AU - McDonald, T. J.

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    N2 - Extracts of human term amnion, placenta, and chorion/decidual tissue (n = 5) contained gastrin-releasing peptide-like immunoreactivity (GRPLI) in amounts of 4.7 ± 2.9 (pmol/g wet wt; mean ± SEM), 3.6 ± 1.1 and 2.9 ± 1.5, respectively. Using C-terminally directed antisera and gel filtration chromatography and reverse-phase high-performance liquid chromatography (HPLC), each tissue contained molecular forms consistent with the presence of GRP1-27 and GRP18-27 but also contained larger amounts of two GRPLI peaks, which apparently are novel GRP-like peptides. In contrast, tissue extracts of human fetal lung contained only GRP1-27, GRP14-27, and GRP18-27. Using RT-PCR and specific GRP primers and probes, messenger RNA encoding for GRP was readily demonstrable from 6-weeks gestation throughout pregnancy to term in full-thickness membranes, placental villi, and decidua. Positive immunohistochemical staining for GRP occurred in extravillous trophoblasts in decidual septa and fetal membranes, cytotrophoblasts, syncytiotrophoblast, and certain stromal cells in placental villi and amniotic epithelium. GRPLI and GRP messenger RNA were present from the earliest dates examined (6-9 weeks) throughout pregnancy to term. Given the proven trophic nature of GRP and related peptides, these peptides may play important roles in maternal, placental, and fetal development during human pregnancy.

    AB - Extracts of human term amnion, placenta, and chorion/decidual tissue (n = 5) contained gastrin-releasing peptide-like immunoreactivity (GRPLI) in amounts of 4.7 ± 2.9 (pmol/g wet wt; mean ± SEM), 3.6 ± 1.1 and 2.9 ± 1.5, respectively. Using C-terminally directed antisera and gel filtration chromatography and reverse-phase high-performance liquid chromatography (HPLC), each tissue contained molecular forms consistent with the presence of GRP1-27 and GRP18-27 but also contained larger amounts of two GRPLI peaks, which apparently are novel GRP-like peptides. In contrast, tissue extracts of human fetal lung contained only GRP1-27, GRP14-27, and GRP18-27. Using RT-PCR and specific GRP primers and probes, messenger RNA encoding for GRP was readily demonstrable from 6-weeks gestation throughout pregnancy to term in full-thickness membranes, placental villi, and decidua. Positive immunohistochemical staining for GRP occurred in extravillous trophoblasts in decidual septa and fetal membranes, cytotrophoblasts, syncytiotrophoblast, and certain stromal cells in placental villi and amniotic epithelium. GRPLI and GRP messenger RNA were present from the earliest dates examined (6-9 weeks) throughout pregnancy to term. Given the proven trophic nature of GRP and related peptides, these peptides may play important roles in maternal, placental, and fetal development during human pregnancy.

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